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EasySep™ Human Naïve Pan T Cell Isolation Kit

Immunomagnetic negative selection kit

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From: 756 USD

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Immunomagnetic negative selection kit
From: 756 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Naïve Pan T Cell Isolation Kit is designed to isolate naïve pan T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages:
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
Components:
    • EasySep™ Human Naïve Pan T Cell Isolation Kit (Catalog #17961)
        • EasySep™ Human Naïve Pan T Cell Isolation Cocktail, 1 mL
        • EasySep™ Human TCR Gamma/Delta Depletion Cocktail, 1 mL
        • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Human Naïve Pan T Cell Isolation Kit (Catalog #17961RF)
        • EasySep™ Human Naïve Pan T Cell Isolation Cocktail, 1 mL
        • EasySep™ Human TCR Gamma/Delta Depletion Cocktail, 1 mL
        • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
        • RoboSep™ Buffer (Catalog #20104)
        • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(7)
Brochure
T Cell Reagents for Your Cellular Therapy Research
Brochure
Tools For Your Immunology Research
Brochure
Cell Enrichment Prior to Cell Sorting
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Antigen Processing and Presentation
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization

Data and Publications

Data

Starting with fresh PBMCs, the naïve pan T cell content (CD3+CD45RA+CD45RO-CD197+) of the isolated fraction is typically 96.1 ± 2.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 4.5% and 97%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.