EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit

Immunomagnetic positive selection of human CD9+ EVs from plasma, serum, cell culture conditioned medium, and urine

EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit

Immunomagnetic positive selection of human CD9+ EVs from plasma, serum, cell culture conditioned medium, and urine

From: 479 USD
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Immunomagnetic positive selection of human CD9+ EVs from plasma, serum, cell culture conditioned medium, and urine
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Product Advantages


  • Fast, easy to use, and column-free.

  • Avoid the need for ultracentrifugation and associated time consuming EV isolation methods.

  • Obtain highly purified EVs without contaminating biofluid components.

What's Included

  • EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit (Catalog #17894)
    • EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Cocktail 1 x 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1 mL
    • NOTE: Isolated extracellular vesicles should not be released from the EasySep™ Releasable Rapidspheres™ 50201

Overview

Easily isolate highly purified human CD9+ extracellular vesicles (EVs) from plasma, serum, cell culture conditioned medium, and urine samples, using immunomagnetic positive selection, with the EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired EVs are labeled with antibody complexes recognizing the specific tetraspanin marker CD9 and magnetic particles. Labeled EVs are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted biofluid components. The EVs of interest remain in the tube. Following magnetic EV isolation in less than 30 minutes, the desired CD9+ EVs are ready for downstream applications such as DNA/RNA extraction, western blot, or mass spectrometry. This method yields EV preparations with low levels of contaminating proteins such as apolipoproteins and albumin, helping to ensure cleaner samples for sensitive downstream analyses.


Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
Other
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation, Extracellular Vesicle Isolation
Brand
EasySep
Area of Interest
Extracellular Vesicle Research

Data Figures

Positive isolation of EVs with the CD9 tetraspanin marker from human plasma and MSC-conditioned medium.

Figure 1. Typical Western Blot Analyses of EVs Isolated from Human plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium

The Western blot analyses in the above examples show positive isolation of EVs with the CD9 tetraspanin marker from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.

Figure 2. Tetraspanin Protein Expression and Recovery of EVs Using EasySep™ Human Pan/CD9/CD63/CD81 Extracellular Vesicle Positive Selection Kits

EVs were isolated from plasma using either EasySep™ Human Extracellular Vesicle Positive Selection Kits or differential ultracentrifugation (2 x 70 min, 100,000 xg) and isolated fractions were analyzed by western blot for tetraspanin protein expression.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17894
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17894
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17894
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.