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Easily isolate highly purified human CD9+ extracellular vesicles (EVs) from plasma, serum, cell culture conditioned medium, and urine samples, using immunomagnetic positive selection, with the EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired EVs are labeled with antibody complexes recognizing the specific tetraspanin marker CD9 and magnetic particles. Labeled EVs are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted biofluid components. The EVs of interest remain in the tube. Following magnetic EV isolation in less than 30 minutes, the desired CD9+ EVs are ready for downstream applications such as DNA/RNA extraction, western blot, or mass spectrometry. This method yields EV preparations with low levels of contaminating proteins such as apolipoproteins and albumin, helping to ensure cleaner samples for sensitive downstream analyses.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Figure 1. Typical Western Blot Analyses of EVs Isolated from Human plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium
The Western blot analyses in the above examples show positive isolation of EVs with the CD9 tetraspanin marker from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.
Figure 2. Tetraspanin Protein Expression and Recovery of EVs Using EasySep™ Human Pan/CD9/CD63/CD81 Extracellular Vesicle Positive Selection Kits
EVs were isolated from plasma using either EasySep™ Human Extracellular Vesicle Positive Selection Kits or differential ultracentrifugation (2 x 70 min, 100,000 xg) and isolated fractions were analyzed by western blot for tetraspanin protein expression.
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Antibody kit for the detection of extracellular vesicles using CD9, CD63, and CD81 markers
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EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.