EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit

Fast & easy isolation of human extracellular vesicles using immunomagnetic positive selection

20 mL of biofluid
Catalog # 17894
403 USD

Overview

The EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9 and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.​
Advantages
⦁ Fast, easy to use, and column-free.
⦁ Avoid the need for ultracentrifugation and associated time consuming EV isolation methods.
⦁ Obtain highly purified EVs without contaminating biofluid components.
Components
  • EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit (Catalog #17894)
    • EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Cocktail 1 x 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1 mL
    • NOTE: Isolated extracellular vesicles should not be released from the EasySep™ Releasable Rapidspheres™ 50201
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
Other
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Extracellular Vesicle Research

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
Catalog #
17894
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
Catalog #
17894
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
Catalog #
17894
Lot #
All
Language
English

Educational Materials (4)

Brochure
Isolate and Purify Extracellular Vesicles
Protocol
How to Characterize Extracellular Vesicles by Western Blotting
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Webinar
Decoding Intercellular Communication via Hepatocyte-Secreted Extracellular Vesicles
35:31
Decoding Intercellular Communication via Hepatocyte-Secreted Extracellular Vesicles

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Positive isolation of EVs with the CD9 tetraspanin marker from human plasma and MSC-conditioned medium.

Figure 1. Typical Western Blot Analyses of EVs Isolated from Human plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium

The Western blot analyses in the above examples show positive isolation of EVs with the CD9 tetraspanin marker from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.

Figure 2. Tetraspanin Protein Expression and Recovery of EVs Using EasySep™ Human Pan/CD9/CD63/CD81 Extracellular Vesicle Positive Selection Kits

EVs were isolated from plasma using either EasySep™ Human Extracellular Vesicle Positive Selection Kits or differential ultracentrifugation (2 x 70 min, 100,000 xg) and isolated fractions were analyzed by western blot for tetraspanin protein expression.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.