CD34+ Progenitor Reprogramming Kit

Integrated workflow containing tools to isolate and expand CD34+ progenitor cells from peripheral blood and their subsequent reprogramming to iPS cells

CD34+ Progenitor Reprogramming Kit

Integrated workflow containing tools to isolate and expand CD34+ progenitor cells from peripheral blood and their subsequent reprogramming to iPS cells

1 Kit
Catalog #05925
1,581 USD

Overview

The CD34+ Progenitor Reprogramming Kit contains an integrated set of tools and reagents to enrich, expand and reprogram CD34+ progenitor cells from human peripheral blood samples.
Advantages:
• Optimized for enrichment, expansion and reprogramming of CD34+ progenitor cells expanded from peripheral blood samples
• Improved reprogramming efficiency and higher frequency of iPS cell colonies, compared to traditional hES cell medium
• Rapid emergence of large colonies with high quality iPS cell-like morphology facilitates identification and subcloning
• Seamlessly integrates with TeSR™ and STEMdiff™ products for downstream maintenance and differentiation of iPS cell lines
Components:
  • SepMate™-50 (Catalog #15440)
  • Lymphoprep™ (Catalog #07801)
  • Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086)
    • RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, 3 x 2 mL
    • EasySep™ Human CD34 Positive Selection Cocktail, 0.4 mL
    • EasySep™ Dextran RapidSheres™, 1 mL
  • StemSpan™ SFEM II (Catalog #09605)
  • StemSpan™ CD34+ Expansion Supplement (Catalog #02691)
  • ReproTeSR™ (Catalog #05920)
    • ReproTeSR™ Basal Medium
    • ReproTeSR™ 20X Supplement
    • ReproTeSR™ 500X Supplement
Subtype:
Specialized Media
Cell Type:
Pluripotent Stem Cells; Hematopoietic Stem and Progenitor Cells
Species:
Human
Application:
Reprogramming; Cell Culture
Brand:
TeSR; StemSpan
Area of Interest:
Stem Cell Biology
Formulation:
Serum-Free; Defined

Scientific Resources

Educational Materials

(3)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Typical Performance of the EasySep™ Complete Kit for Human Whole Blood CD34+ Cells

Figure 1. Typical Performance of the EasySep™ Complete Kit for Human Whole Blood CD34+ Cells

(A) In whole PB, the proportion of CD34+/CD45+ cells is typically very low (<1%). (B) In the RosetteSep™ enrichment step, unwanted cells are removed during density gradient centrifugation. This step enriches the population of CD34+/CD45+ cells (19.8%) in this example). (C) After positive selection of CD34+ cells using EasySep™, a highly pure population of CD34+/CD45+ cells (96.2% in this example) can be obtained. Values are reported as a percentage of viable CD45+ cells.

StemSpan™ SFEM II and CD34+ Expansion Supplement Support Expansion of CD34+ Cells

Figure 2. StemSpan™ SFEM II and CD34+ Expansion Supplement Support Expansion of CD34+ Cells

TNC: Total Nucleated Cell

Schematic of ReproTeSR™ Reprogramming Timeline

Figure 3. Schematic of ReproTeSR™ Reprogramming Timeline

ReproTeSR™ is used during the entire induction phase of reprogramming (day 3 to 21). On days 3 and 5, ReproTeSR™ is added to StemSpan™ growth media (in a fed-batch manner) to facilitate attachment of transfected cells. Attached cells are further cultured in ReproTeSR™ with daily full media changes until putative iPS cell colonies emerge (days 21-28). iPS cell colonies can then be isolated and propagated in TeSR™ media. (mTeSR™1, TeSR™2, TeSR™-E8™).

Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Figure 4. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Efficiency of reprogramming (A) erythroid cells, or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean +/- SEM, erythroid cells n=4, CD34+ cells n=5.

ReproTeSR™ Generates iPS Cell Colonies With Superior Colony Morphology

Figure 5. ReproTeSR™ Generates iPS Cell Colonies With Superior Colony Morphology

Representative images of iPS cell colonies generated from isolated CD34+ progenitor cells using (A) ReproTeSR™ and (B) hESC) medium. iPS cell colonies produced using ReproTeSR™ exhibit more defined borders, compact morphology and reduced differentiation compared with hESC medium. 200X magnification.

Reprogramming Efficiency of CD34+ and Erythroid Progenitor Cells With ReproTeSR™

Figure 6. Reprogramming Efficiency of CD34+ and Erythroid Progenitor Cells With ReproTeSR™

Average values in bold (range).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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