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Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136

(C3H x BALB/c) F1 hybrid monoclonal IgG2a antibody against mouse NK1.1 (CD161)

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Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136

(C3H x BALB/c) F1 hybrid monoclonal IgG2a antibody against mouse NK1.1 (CD161)

From: 77 USD
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(C3H x BALB/c) F1 hybrid monoclonal IgG2a antibody against mouse NK1.1 (CD161)
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Overview

The PK136 antibody reacts with murine NK1.1 (CD161), an ~80 kDa homodimeric type 2 transmembrane glycoprotein and C-type lectin receptor expressed on NK cells, NK-T cells, and rare subsets of T cells and monocytes in select strains of mice. NK1.1 is encoded by the Klrb1b and Klrb1c genes, which specify CD161b and CD161c polypeptides, respectively. CD161b is expressed only by Swiss NIH and SJL mice, and CD161c by certain strains such as C57BL, FVB/N, and NZB (but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, or 129). NK1.1 has functional roles in modulating several processes, including the activation and proliferation of NK cells, induction of interferon-γ production, and release of cytotoxic granules. Its expression on the cell surface is specifically upregulated by IL-12. For detection of NK cells in NK1.1- strains, Anti-Mouse CD49b Antibody, Clone DX5 (Catalog #60020) is recommended. DX5 recognizes CD49b (integrin α2), another commonly used NK cell marker.
Subtype
Primary Antibodies
Target Antigen
NK1.1 (CD161)
Alternative Names
CD161b, CD161c, Klrb1b, Klrb1c, Ly-55, Ly-59, NK-1.1, NKRP1, NKRP1a, NKRP1b
Reactive Species
Mouse
Conjugation
Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
NK Cells
Species
Mouse
Application
Cell Isolation, CyTOF, Depletion, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
PK136
Gene ID
17059
Isotype
IgG2a, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, APC (Catalog #60071AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, Biotin (Catalog #60071BT), followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, FITC (Catalog #60071FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a mouse IgG2a, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™
Catalog #
60103PB.1, 60103PB
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin
Catalog #
100-0463, 60103BT, 100-0462
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488
Catalog #
60103AD.1, 60103AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC
Catalog #
100-0464, 100-0465, 60103FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE
Catalog #
100-0460, 100-0461
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136
Catalog #
100-0459
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5
Catalog #
60103PS, 60103PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC
Catalog #
60103AZ.1, 60103AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™
Catalog #
60103PB.1, 60103PB
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin
Catalog #
100-0463, 60103BT, 100-0462
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488
Catalog #
60103AD.1, 60103AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC
Catalog #
100-0464, 100-0465, 60103FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE
Catalog #
100-0460, 100-0461
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136
Catalog #
100-0459
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5
Catalog #
60103PS, 60103PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC
Catalog #
60103AZ.1, 60103AZ
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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