Anti-Mouse NK1.1 (CD161), Clone PK136

Mouse monoclonal IgG2a antibody against mouse NK1.1 (CD161)

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Mouse monoclonal IgG2a antibody against mouse NK1.1 (CD161)
From: 65 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The PK136 antibody reacts with murine NK1.1 (CD161), an ~80 kDa homodimeric type 2 transmembrane glycoprotein and C-type lectin receptor expressed on NK cells, NK-T cells, and rare subsets of T cells and monocytes in select strains of mice. NK1.1 is encoded by the Klrb1b and Klrb1c genes, which specify CD161b and CD161c polypeptides, respectively. CD161b is expressed only by Swiss NIH and SJL mice, and CD161c by certain strains such as C57BL, FVB/N, and NZB (but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, or 129). NK1.1 has functional roles in modulating several processes, including the activation and proliferation of NK cells, induction of interferon-γ production, and release of cytotoxic granules. Its expression on the cell surface is specifically upregulated by IL-12. For detection of NK cells in NK1.1- strains, Anti-Mouse CD49b Antibody, Clone DX5 (Catalog #60020) is recommended. DX5 recognizes CD49b (integrin α2), another commonly used NK cell marker.
Subtype:
Primary Antibodies
Target Antigen:
NK1.1 (CD161)
Alternative Names:
CD161b, CD161c, Klrb1b, Klrb1c, Ly-55, Ly-59, NK-1.1, NKRP1, NKRP1a, NKRP1b
Reactive Species:
Mouse
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated; Pacific Blue
Host Species:
Mouse
Cell Type:
NK Cells
Application:
CyTOF; Depletion; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Cell Isolation
Area of Interest:
Immunology
Clone:
PK136
Gene ID:
17059
Isotype:
IgG2a, kappa

Scientific Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language
Product Information Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136
60103, 60103.1
All
EN
Product Information Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, Alexa Fluor® 488
60103AD, 60103AD.1
All
EN
Product Information Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, APC
60103AZ, 60103AZ.1
All
EN
Product Information Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, Biotin
60103BT, 60103BT.1
All
EN
Product Information Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, FITC
60103FI, 60103FI.1
All
EN
Product Information Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, PE
60103PE, 60103PE.1
All
EN
Product Information Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, PerCP-Cy5.5
60103PS, 60103PS.1
All
EN
Product Information Sheet
Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™
60103PB, 60103PB.1
All
EN
Safety Data Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136
60103, 60103.1
All
English
Safety Data Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, Alexa Fluor® 488
60103AD, 60103AD.1
All
English
Safety Data Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, APC
60103AZ, 60103AZ.1
All
English
Safety Data Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, Biotin
60103BT, 60103BT.1
All
English
Safety Data Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, FITC
60103FI, 60103FI.1
All
English
Safety Data Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, PE
60103PE, 60103PE.1
All
English
Safety Data Sheet
Anti-Mouse NK1.1 (CD161), Clone PK136, PerCP-Cy5.5
60103PS, 60103PS.1
All
English

Educational Materials

(4)
Brochure
Complete Antibody Listing
Brochure
Tools For Your Immunology Research
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Dendritic Cell Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization
Natural Killer Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, APC (Catalog #60071AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, Biotin (Catalog #60071BT), followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, FITC (Catalog #60071FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a mouse IgG2a, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.