Anti-Mouse IFN-gamma Antibody, Clone XMG1.2

Rat monoclonal IgG1 antibody against mouse IFN-gamma
Catalog #
100-0277_C
Rat monoclonal IgG1 antibody against mouse IFN-gamma
From: 62 USD

Overview

The XMG1.2 antibody reacts with mouse interferon-gamma (IFN-γ), a glycosylated type II interferon comprising a homodimer of ~20 kDa subunits in its active form. IFN-γ is produced primarily by activated T lymphocytes and natural killer (NK) cells in response to antigens, including alloantigens, mitogens, and enterotoxins. IFN-γ binds with high affinity to the α subunit of the IFN-γ receptor, resulting in the ligand-induced assembly of a tetrameric α2β2 receptor complex capable of mediating the cellular response to the cytokine through activation of the JAK-STAT pathway. The IFN-γ receptor is expressed on the surface of all nucleated cells. IFN-γ has important functions in a wide range of biological processes, including macrophage activation, mediating the immune response to viruses and bacteria, and modulating cellular proliferation and apoptosis. The XMG1.2 antibody is suitable for detection of intracellular IFN-γ by methods such as flow cytometry, when paired with an appropriate secondary antibody. The XMG1.2 antibody is also widely used in various assays to neutralize the functional activity of IFN-γ.
Subtype
Primary Antibodies
Target Antigen
IFNγ
Alternative Names
IFN-γ, Immune interferon, Interferon-gamma, Macrophage-activating factor (MAF), T cell interferon, Type II interferon
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, PE
Host Species
Rat
Cell Type
Dendritic Cells, Macrophages, NK Cells, T Cells
Application
ELISA, ELISpot, Flow Cytometry
Area of Interest
Immunology
Clone
XMG1.2
Gene ID
15978
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Catalog #
100-0277, 100-0278
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE
Catalog #
100-0279, 100-0280
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC
Catalog #
100-0281, 100-0282
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, Biotin
Catalog #
100-0283, 100-0284
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Catalog #
100-0277, 100-0278
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE
Catalog #
100-0279, 100-0280
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC
Catalog #
100-0281, 100-0282
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, Biotin
Catalog #
100-0283, 100-0284
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Brochure
Tools For Your Immunology Research

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.

Figure 1. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC

Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, Biotin.

Figure 2. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, Biotin

Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-γ Antibody, Clone XMG1.2, Biotin, followed by streptavidin (SAV) PE.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody, Clone XMG1.2, FITC.

Figure 3. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC

Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.

Figure 4. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE

Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.

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