Anti-Human CD8a Antibody, Clone SK1

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a
Anti-Human CD8a Antibody, Clone SK1, PE

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a, PE-conjugated

100 Tests
Catalog # 60125PE
501 USD
Anti-Human CD8a Antibody, Clone SK1, PE

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a, PE-conjugated

25 Tests
Catalog # 60125PE.1
203 USD

Overview

The SK1 antibody reacts with CD8a, a 32 - 34 kDa type I transmembrane glycoprotein that is a subunit of CD8. CD8 is a disulfide-bonded dimer, found either as a heterodimer of CD8a (α) and CD8b (β) subunits (i.e. αβ) or a homodimer (αα). CD8 acts as a co-receptor to the T cell receptor (TCR) during T cell activation by binding MHC class I molecules presented by an antigen-presenting cell. It functions to strengthen the association between the TCR and MHC I-antigen complex and to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. The CD8a chain binds to the α3 domain of class I MHC molecules. CD8 is expressed by a majority of thymocytes, a subset of mature peripheral blood T cells (T cytotoxic cells), a proportion of β T cells, and at lower levels by NK cells (which predominantly express CD8a homodimers). Reportedly, the SK1 antibody exhibits a blocking effect on MHC:peptide tetramer binding to TCR and also blocks proliferation of Leu-2 T cells in mixed leukocyte reactions (MLR).
Subtype
Primary Antibodies
Target Antigen
CD8a
Reactive Species
Human
Conjugation
PE
Host Species
Mouse
Cell Type
T Cells, T Cells, CD8+
Species
Human
Application
Cell Isolation, Flow Cytometry
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD8a Antibody, Clone SK1, PE
Catalog #
60125PE, 60125PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD8a Antibody, Clone SK1, PE
Catalog #
60125PE, 60125PE.1
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

Figure 1. Data for PE Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone SK1, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE; solid line histogram).

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