Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6

Mouse monoclonal antibody against human, rhesus, cynomolgus CD81, unconjugated
Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6

Mouse monoclonal antibody against human, rhesus, cynomolgus CD81, unconjugated

100 µg
Catalog # 100-0209
294 USD

Overview

The 5A6 antibody reacts with an epitope located in the large extracellular loop (EC2) of human CD81 (TAPA-1). CD81 exhibits broad expression, including on the surface of B and T cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It is also a marker characteristic of extracellular vesicles.

CD81, a 26 kDa non-glycosylated type III transmembrane protein, belongs to the tetraspanin family. Tetraspanins contain four transmembrane domains, two extracellular loops, and short cytoplasmic N- and C-termini. CD81 associates with numerous integrins, co-receptors, and other proteins to form multimolecular complexes in the plasma membrane called tetraspanin-enriched microdomains. Palmitoylation of the juxtamembrane cysteine residues is important for its association with other proteins and the plasma membrane.

CD81 modulates a diverse array of biological functions including cell-cell adhesion and fusion, cell migration, and proliferation. For example, together with CD19 and CD21, CD81 forms a co-receptor for the B cell receptor on B cells. The epitope of the 5A6 antibody is hidden when CD81 is in complex with CD19 but accessible following activation of the B cell. Mutations in CD81 have been associated with the immune disorder common variable immunodeficiency (CVID). CD81 has also been implicated in mediating the entry of hepatitis C virus into cells.
Subtype
Primary Antibodies
Target Antigen
CD81
Alternative Names
CVID6
Reactive Species
African Green Monkey, Baboon, Cynomolgus, Rhesus, Chimpanzee, Human
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Other
Application
Flow Cytometry, Western Blotting
Area of Interest
Extracellular Vesicle Research
Clone
5A6
Gene ID
975
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6
Catalog #
100-0209
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6
Catalog #
100-0209
Lot #
All
Language
English

Educational Materials (5)

Brochure
Isolate and Purify Extracellular Vesicles
Protocol
How to Characterize Extracellular Vesicles by Western Blotting
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Webinar
Decoding Intercellular Communication via Hepatocyte-Secreted Extracellular Vesicles
35:31
Decoding Intercellular Communication via Hepatocyte-Secreted Extracellular Vesicles
Webinar
Online Immunology Journal Club: Viral Exploitation of Extracellular Vesicles to Spread Infection
23:18
Online Immunology Journal Club: Viral Exploitation of Extracellular Vesicles to Spread Infection

Data and Publications

Data

Data showing western blot analysis of extracellular vesicles isolated from mesenchymal stromal cell-conditioned medium with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6 and flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6.

Figure 1. Analysis of Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6 with Western Blot and Flow Cytometry

Extracellular vesicles (EVs) isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column were analysed with (A) western blot analysis with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes were gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin and then (B) labeled with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6, followed by a goat anti-mouse IgG1 antibody, APC.

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