Agar Leukocyte Conditioned Medium

Source of colony-stimulating factors for assays of human hematopoietic progenitor cells

Agar Leukocyte Conditioned Medium

Source of colony-stimulating factors for assays of human hematopoietic progenitor cells

From: 571 USD
Catalog #
02300_C
Source of colony-stimulating factors for assays of human hematopoietic progenitor cells

Overview

Agar Leukocyte Conditioned Medium (Agar-LCM) is a source of colony-stimulating factors for assays of hematopoietic progenitor cells from light-density fractions of human bone marrow and peripheral blood. It is suitable for the growth of colony-forming unit erythroid (CFU­E), burst-forming unit-erythroid (BFU-E), colony-forming unit-macrophage (CFU-M), colony-forming unit-granulocyte (CFU-G), colony-forming unit-granulocyte, macrophage (CFU-GM) and colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM) when added to MethoCult™ medium at a final concentration of 10% with the addition of erythropoietin (EPO; Catalog #02625).

Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium.
Contains
• Agar Leukocyte Conditioned Medium
• Fetal bovine serum (10%)
• Iscove's MDM
• 1 x 10^4 2-Mercaptoethanol (ME)
Subtype
Basal Media
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Application
Cell Culture
Area of Interest
Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
02300
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
02300
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

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