RosetteSep™ DM-L Density Medium
Density gradient medium

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Overview
Please Note: In light of the new EU Regulation 2017/746 (IVDR) of the European Parliament and of the Council on in vitro diagnostic medical devices, which is expected to become effective in 2022, STEMCELL recently deregistered and removed the CE IVD claims associated with these products, and they are now labeled “For Research Use Only”. Please note there have been no changes to the form, function and quality assurances associated with these products. For more information please contact the Quality Assurance and Regulatory Department at qaschangenote@stemcell.com.
• Hetastarch
• Sodium chloride
• Sodium lactate
• Dextrose
• Calcium chloride
• Potassium chloride
• Magnesium chloride
• Water
Data Figures

Figure 1. Minimal Granulocyte Contamination Using RosetteSep™ DM-L Density Medium
Granulocyte contamination is typically less than 1% when enriching lymphocytes from fresh whole blood using RosetteSep™. There was no significant difference in the level of granulocyte contamination when using RosetteSep™ DM-L in place of Ficoll-paque PLUS to enrich lymphocytes (N=11)

Figure 2. Rosettesep™ DM-L Improves the Recovery of Lymphocytes Enriched with Rosettesep™ from Fresh Blood

Figure 3. Cell Purity Is Equivalent or Higher for Rosettesep™ Lymphocyte Enrichment Using Rosettesep™ DM-L Instead of Ficoll-paque®
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Frequently Asked Questions
What is RosetteSep™?
How does RosetteSep™ work?
What factors affect cell recovery?
Which cell samples can RosetteSep™ be used with?
Can RosetteSep™ be used with previously frozen or cultured cells?
Can RosetteSep™ be used to enrich progenitors from cord blood?
Does RosetteSep™ work with mouse cells?
Which anticoagulant should be used with RosetteSep™?
Should the anticoagulant be washed off before using RosetteSep™?
Publications (1)
Abstract
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.