Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2

Rat monoclonal IgG2b antibody against mouse MHC Class II (I-A/I-E)
Catalog #
100-0290_C
Rat monoclonal IgG2b antibody against mouse MHC Class II (I-A/I-E)
From: 52 USD

Overview

The M5/114.15.2 (M5/114) antibody reacts with an extracellular epitope on the mouse major histocompatibility complex class II receptor (MHC-II). MHC-II is a heterodimeric transmembrane glycoprotein comprising an α subunit associated non-covalently with a β subunit. In mice, MHC-II complexes are designated I-A and I-E and comprise Aα/Aβ and Eα/Eβ heterodimers, respectively. Together with the CD3/T cell receptor (TCR) complex and CD4 molecules, MHC-II mediates a critical function in presenting peptides generated from hydrolysis of exogenous antigens by antigen-presenting cells to CD4+ T (helper) cells, thereby either suppressing or inducing an immune response to the peptides. The M5/114.15.2 antibody recognizes a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek (but not I-Af, I-Ak, or I-As) MHC class II alloantigens expressed on B cells, dendritic cells, macrophages, monocytes, and activated T cells of mice carrying the H-2b, d, p, q, r, and u haplotypes (but not haplotypes H-2f or H-2s). The M5/114 antibody reportedly inhibits I-A-restricted responses by T cells of the H-2b, d, q, and u (but not H-2f, k, or s) haplotypes. It does not react with cells from NOD (H-2g7) mice.
Subtype
Primary Antibodies
Target Antigen
MHC Class II (I-A/I-E)
Alternative Names
IA, I-A, IA/IE, I-A/E, I-E, MHC class II, MHC-II
Reactive Species
Mouse
Conjugation
APC, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
B Cells, Macrophages, Monocytes, T Cells
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blotting
Area of Interest
Immunology
Clone
M5/114.15.2
Gene ID
14961/14969
Isotype
IgG2b, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, APC
Catalog #
100-0290, 100-0291
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, PE
Catalog #
100-0292, 100-0293
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, FITC
Catalog #
100-0294, 100-0295, 100-0296
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2
Catalog #
100-0297
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, APC
Catalog #
100-0290, 100-0291
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, PE
Catalog #
100-0292, 100-0293
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, FITC
Catalog #
100-0294, 100-0295, 100-0296
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2
Catalog #
100-0297
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC or a rat IgG2b, kappa APC isotype control antibody.

Figure 1. Data for Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC (filled histogram) or a rat IgG2b, kappa APC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC or a rat IgG2b, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, FITC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC (filled histogram) or a rat IgG2b, kappa FITC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE or a rat IgG2b, kappa PE isotype control antibody.

Figure 3. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, PE

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE.

Figure 4. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2,Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE (filled histogram) or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE (solid line histogram).

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