Anti-Human CD45RA, Clone HI100

Mouse monoclonal IgG2b antibody against human, chimpanzee CD45RA
Catalog #
100-0316_C
Mouse monoclonal IgG2b antibody against human, chimpanzee CD45RA
From: 52 USD

Overview

The HI100 antibody reacts with an extracellular epitope on the CD45RA isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5, and 6 that encode the extracellular RA, RB, and RC polypeptides of CD45 gives rise to several isoforms with molecular masses of 180 - 240 kDa. Excision of exon 4 generates the ~220-kDa CD45RA isoform, which is expressed on naïve T cells, subsets of B cells and monocytes, and medullary thymocytes. CD45RA functions to enhance signaling through both the T cell receptor and B cell receptor. The HI100 antibody is commonly used in combination with antibodies against the CD45RO isoform to discern naïve and memory T cells and is useful for identifying the suppressor/inducer subset of CD4+ lymphocytes. The proportion of CD45RA+ T cells typically decreases with age.
Subtype
Primary Antibodies
Target Antigen
CD45RA
Alternative Names
B220, CD45, GP180, Lyt-4, Ly-5, LY5, L-CA, LCA, Protein tyrosine phosphatase receptor type C, PTPRC, T200
Reactive Species
Chimpanzee, Human
Conjugation
APC-Cyanine7, Biotin, PE, Unconjugated
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Leukocytes, Lymphocytes
Application
CyTOF, ELISA, FACS, Flow Cytometry, Functional Assay, Immunoaffinity Chromatography, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blotting
Area of Interest
Immunology
Clone
HI100
Gene ID
5788
Isotype
IgG2b, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100, APC-Cyanine7
Catalog #
100-0316, 100-0317
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100
Catalog #
100-0318
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100, PE
Catalog #
100-0319, 100-0320
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RA, Clone HI100, Biotin
Catalog #
100-0321, 100-0322
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100, APC-Cyanine7
Catalog #
100-0316, 100-0317
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100
Catalog #
100-0318
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100, PE
Catalog #
100-0319, 100-0320
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RA, Clone HI100, Biotin
Catalog #
100-0321, 100-0322
Lot #
All
Language
English

Product Applications

Data and Publications

Data

Figures showing flow cytometry analysis of human peripheral blood mononuclear cells (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, clone MPC-11, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 or a mouse IgG2b, kappa APC-Cyanine7 isotype control antibody.

Figure 1. Data for Anti-Human CD45RA Antibody, Clone HI100, APC-Cy7

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, clone MPC-11, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 (filled histogram) or a mouse IgG2b, kappa APC-Cyanine7 isotype control antibody (solid line histogram).

Figures showing flow cytometry analysis of (A) human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by streptavidin (SAV) PE, and anti-human CD45RO antibody, clone UCHL1, APC,(B) human PBMCs labeled with a mouse IgG2b, kappa Biotin isotype control antibody followed by SAV PE, and anti-human CD45RO antibody, clone UCHL1, APC, and (C) human PBMCs labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by SAV PE or a mouse IgG2b, kappa Biotin isotype control antibody, followed by SAV PE.

Figure 2. Data for Anti-Human CD45RA Antibody, Clone HI100, Biotin

Flow cytometry analysis of human PBMCs (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by streptavidin (SAV) PE, and anti-human CD45RO antibody, clone UCHL1, APC. (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2b, kappa Biotin isotype control antibody followed by SAV PE, and anti-human CD45RO antibody, clone UCHL1, APC. (C) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by SAV PE (filled histogram) or a mouse IgG2b, kappa Biotin isotype control antibody, followed by SAV PE (solid line histogram). Viable lymphocytes were gated for analysis.

Figures showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa PE isotype control antibody and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE or a mouse IgG2b, kappa PE isotype control antibody.

Figure 3. Data for Anti-Human CD45RA Antibody, Clone HI100, PE

Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa PE isotype control antibody and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE (filled histogram) or a mouse IgG2b, kappa PE isotype control antibody (solid line histogram).

Figures showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, Clone MPC-11, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE or a mouse IgG2b, kappa isotype control antibody followed by a goat anti-mouse IgG2b, PE.

Figure 4. Data for Anti-Human CD45RA Antibody, Clone HI100, Unconjugated

Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, Clone MPC-11, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC,and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE (filled histogram) or a mouse IgG2b, kappa isotype control antibody followed by a goat anti-mouse IgG2b, PE (solid line histogram).

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.