RoboSep™-C Human CD4+ T Cell Isolation Kit

Immunomagnetic negative selection kit
RoboSep™-C Human CD4+ T Cell Isolation Kit

Immunomagnetic negative isolation of untouched CD4+ T cells from leukopaks

1 x 10^10 cells
Catalog # 100-0200
Required Products
  1. RoboSep™-C  Cell Isolation Instrument
    RoboSep™-C

    Automated closed-system immunomagnetic cell isolation instrument

  2. RoboSep™-C Tubing Set
    RoboSep™-C Tubing Set

    For automated cell isolation with RoboSep™-C

  3. RoboSep™-C PBS/EDTA Buffer
    RoboSep™-C PBS/EDTA Buffer

    Cell separation buffer

Overview

The RoboSep™-C Human CD4+ T Cell Isolation Kit is designed to isolate untouched and highly purified CD4+ T cells from fresh human peripheral blood leukopaks using RoboSep™-C (Catalog #100-0185 and #100-0186) for automated immunomagnetic negative selection.

This kit targets non-CD4+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Advantages
⦁ Fully automated large-scale isolation in a closed system
⦁ Up to 98% purity with high recovery
⦁ Untouched, viable cells
Components
  • RoboSep™-C Human CD4+ T Cell Isolation Cocktail, 10 mL
  • RoboSep™-C Dextran RapidSpheres™ 30106, 10 mL
Magnet Compatibility
• RoboSep™-C (Catalog #400-2274)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Cell Therapy, Drug Discovery and Toxicity Testing, Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
RoboSep™-C Human CD4+ T Cell Isolation Kit
Catalog #
100-0200
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™-C Human CD4+ T Cell Isolation Kit
Catalog #
100-0200
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™-C Human CD4+ T Cell Isolation Kit
Catalog #
100-0200
Lot #
All
Language
English

Educational Materials(5)

Brochure
T Cell Reagents for Your Cellular Therapy Research
Brochure
Tools For Your Immunology Research
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Wallchart
Production of Chimeric Antigen Receptor T Cells
Video
0:59
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Typical RoboSep™-C Human CD4+ T Cell Isolation Profile

Starting from a fresh human peripheral blood leukopak, the CD4+ T cell content (CD3+CD4+) of the isolated fraction is typically 95.7 ± 2.8% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 31.5% and 95.4%, respectively.

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