Anti-Mouse SSEA-3 Antibody, Clone MC-631

Rat monoclonal IgM antibody against human, mouse, rat SSEA-3
Catalog #
60061_C
Rat monoclonal IgM antibody against human, mouse, rat SSEA-3
From: 197 USD

Overview

The MC-631 antibody reacts with a galactosylgloboside epitope on the stage-specific embryonic antigen-3 (SSEA-3), which is expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), undifferentiated embryonic stem (ES), and induced pluripotent stem (iPS) cells, as well as rhesus monkey ES cell lines. No immunoreactivity is evident with undifferentiated mouse EC, EG and ES cells. Expression of SSEA-3 is down regulated following differentiation of ES and EC cells. In contrast, the differentiation of mouse ES and EC cells may be accompanied by an increase in SSEA-3 expression.

This antibody clone has been verified for labeling human ES and iPS cells grown in TeSR™-E8™ (Catalog #05940), mTeSR™1 (Catalog #85850) and TeSR™2 (Catalog #05860) and has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit (Catalog #18166).
Subtype
Primary Antibodies
Target Antigen
SSEA-3
Alternative Names
Stage-specific embryonic antigen-3
Reactive Species
Rhesus, Human, Mouse, Rat
Conjugation
Alexa Fluor 488, PE, Unconjugated
Host Species
Rat
Cell Type
Pluripotent Stem Cells
Species
Human, Mouse, Rat, Non-Human Primate
Application
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Area of Interest
Stem Cell Biology
Clone
MC-631
Gene ID
93961
Isotype
IgM, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631
Catalog #
60061.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488
Catalog #
60061AD, 60061AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE
Catalog #
60061PE, 60061PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631
Catalog #
60061.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488
Catalog #
60061AD, 60061AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE
Catalog #
60061PE, 60061PE.1
Lot #
All
Language
English

Educational Materials (3)

Brochure
Products for Human Pluripotent Stem Cells
Brochure
Standardized Solutions for Mouse Pluripotent Stem Cells
Brochure
Reliable Antibodies for Your Research

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or fibroblasts (negative control; dashed line histogram) labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, followed by Goat Anti-Rat IgM (Heavy Chain) Antibody, Polyclonal, APC (Catalog #60140AZ). Labeling of human ES cells with a rat IgM isotype control antibody, followed by Goat Anti-Rat IgM (Heavy Chain) Antibody, Polyclonal, APC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Inset shows cells labeled with a rat IgM, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiMouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488. Labeling of human ES cells with a rat IgM, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488. Inset shows cells labeled with a rat IgM, kappa Alexa Fluor® 488 isotype control antibody. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488 (filled histogram) or a rat IgM, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiMouse SSEA-3 Antibody, Clone MC-631, PE. Labeling of human ES cells with a rat IgM, kappa PE isotype control antibody is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE. Inset shows cells labeled with a rat IgM, kappa PE isotype control antibody. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE (filled histogram) or a rat IgM, kappa PE isotype control antibody (solid line histogram).

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.