EasySep™ Mouse F4/80 Positive Selection Kit

Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage

EasySep™ Mouse F4/80 Positive Selection Kit

Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage

EasySep™ Mouse F4/80 Positive Selection Kit
2 x 10^9 cells
590 USD
Catalog # 100-0659

Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage

Product Advantages

⦁ Fast and easy-to-use
⦁ Up to 90% purity
⦁ No columns required
⦁ Isolated cells are not fluorochrome-labeled

What's Included

  • EasySep™ Mouse F4/80 Positive Selection Component A, 0.3 mL
  • EasySep™ Mouse F4/80 Positive Selection Component B, 0.6 mL
  • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
  • Normal Rat Serum, 2 mL

Overview

Easily isolate F4/80+ macrophages in as little as 24 minutes—without the use of columns—from mouse splenocytes, lung tissue, and peritoneal lavage.

Using immunomagnetic positive selection, F4/80+ cells are labeled with antibodies and dextran-coated magnetic particles (RapidSpheres™), for column-free cell separation using an EasySep™ magnet. Isolated cells are highly purified and ready for your downstream applications.

Visit www.EasySep.com to learn more about column-free EasySep™ cell separation technology.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Macrophages
Species
Mouse
Sample Source
Lung, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0659
Lot #
All
Language
English
Catalog #
100-0659
Lot #
All
Language
English
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0659
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

FACS plot showing cell populations from lung tissue pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 1. Typical Profile for F4/80+ Cells Isolated from Lung Tissue Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.

FACS plot showing cell populations from peritoneal lavage pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 2. Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 94.4 ± 2.9% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 40.9% and 94.1%, respectively.

FACS plot showing cell populations from spleen pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 3. Typical Profile for F4/80+ Cells Isolated from Spleen Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.