Anti-Mouse TER119 Antibody, Clone TER-119

Rat (WI) monoclonal IgG2b antibody against mouse TER119

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Rat (WI) monoclonal IgG2b antibody against mouse TER119
From: 59 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The TER-119 antibody reacts with murine TER119 (Ly-76), an ~52 kDa protein associated with glycophorin A on the surface of cells of the erythroid lineage in embryonic yolk sac, fetal and newborn liver, adult bone marrow, peripheral blood, and lymphoid organs. TER119 is an erythroid-specific marker expressed at all stages of differentation from early proerythroblasts to mature erythrocytes, but not by erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid). The TER-119 antibody is a component of the "lineage cocktail" used to detect, or deplete cells committed to hematopoietic lineages. In adult mice, TER119 is found on ~20 - 25% of bone marrow cells and ~2 - 3% of splenocytes.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852).
Subtype:
Primary Antibodies
Target Antigen:
TER119
Alternative Names:
Ly-76, TER-119
Reactive Species:
Mouse
Conjugation:
Biotin; FITC; PE; PerCP-Cyanine5.5
Host Species:
Rat
Cell Type:
Erythroid Cells; Red Blood Cells
Application:
Cell Isolation; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Immunology
Clone:
TER-119
Gene ID:
104231
Isotype:
IgG2b, kappa

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Complete Antibody Listing
Brochure
Tools For Your Immunology Research
Brochure
Cell Culture Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Dendritic Cell Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization
Hematopoietic Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Expansion and Differentiation
Analysis

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, followed by goat antimouse IgG, FITC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Image showing flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE.

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE (filled histogram) or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 5. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.