Human Recombinant CD40 Ligand

Cluster of differentiation 40 ligand
Catalog #
78162_C
Cluster of differentiation 40 ligand
From: 179 EUR

Overview

CD40 ligand is a type II transmembrane glycoprotein that belongs to the tumor necrosis factor (TNF) superfamily (Quezada et al.). CD40 ligand forms a bioactive homotrimer that exists as both soluble and membrane-bound forms (Khandekar et al.). CD40 ligand is expressed on T cells, monocytes, basophils, eosinophils, platelets, dendritic cells, and endothelial cells. Its receptor, CD40, is expressed on B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (van Kooten & Banchereau). Binding of CD40 ligand to CD40 stimulates B cell proliferation, immunoglobulin class switching, antibody secretion, and T cell-dependent humoral responses. Dysregulation of CD40 ligand contributes to immune deficiency in HIV and AIDS (Rickert et al.). CD40 ligand has also been linked to the pathology of atherosclerosis, atherothrombosis, and restenosis (Hassan et al.).
Subtype
Cytokines
Alternative Names
CD154, CD40L, CD40 antigen ligand, hCD40L, HIGM1, hyper IgM syndrome, IMD3, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
Cell Type
B Cells, Dendritic Cells, Endothelial Cells, Granulocytes and Subsets, Monocytes, T Cells
Species
Human
Molecular Weight
16.3 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant CD40 Ligand
Catalog #
78162, 78162.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant CD40 Ligand
Catalog #
78162, 78162.1
Lot #
All
Language
English

Educational Materials (3)

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant CD40 Ligand was tested by its ability to induce alkaline phosphatase activity in HEKBlue ™ CD40L responsive indicator cells. Alkaline phosphatase activity was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the ligand at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the example above is 16.7 ng/mL. (B) 1 μg of Human Recombinant CD40 Ligand was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant CD40 Ligand has a predicted molecular mass of 16.3 kDa.

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