Mouse TNF-alpha ELISA Kit

For detection and measurement of mouse tumor necrosis factor-alpha

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From: 575 USD

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For detection and measurement of mouse tumor necrosis factor-alpha
From: 575 USD

Overview

The Mouse Tumor Necrosis Factor-alpha (TNF-α) ELISA Kit is designed for the quantitative detection and measurement of mouse TNF-α in biological fluids such as serum, plasma, and cell culture supernatants. TNF-α is primarily produced by activated T cells and macrophages in response to inflammation or infection, but is also produced by B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α is a pro-inflammatory cytokine and stimulates anti-tumor responses in vitro.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Components:
  • Mouse TNF-alpha ELISA Kit, 2 plates (Catalog #02030)
    • Mouse TNF-α ELISA Plate, 2x
    • Mouse TNF-α Standard, 1 vial
    • Mouse TNF-α Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Mouse TNF-alpha ELISA Kit, 10 plates (Catalog #02031)
    • Mouse TNF-α  ELISA Plate, 10x
    • Mouse TNF-α Standard, 1 vial
    • Mouse TNF-α Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x
Subtype:
Complete Kits
Cell Type:
B Cells; Cardiomyocytes, PSC-Derived; Dendritic Cells; Endothelial Cells; Granulocytes and Subsets; Macrophages; Mouse Embryonic Fibroblasts; Myogenic Stem and Progenitor Cells; NK Cells; T Cells
Species:
Mouse
Application:
ELISA
Area of Interest:
Immunology; Stem Cell Biology

Scientific Resources

Educational Materials

(2)

Data and Publications

Data

Representative Standard Curve

• Reportable range: 15.6 - 2000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 5 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 88/532.
• Recovery: A mid-curve recovery of 84 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.4% (CV). The Inter-assay precision of this assay is 3.7% (CV).

* National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.