Table 1. HSC Expansion Culture with CD34⁺ Human Cord Blood Cells Cultured in StemSpan™ SFEM Containing CD34⁺ Expansion Supplement

Shown are the percent CD34⁺ cells, fold expansion of total nucleated cells (TNC) and CD34⁺ cells, and numbers of colony-forming units (CFU) produced per input CD34⁺ cell after 7 days of hsc expansion culture of enriched CD34⁺ cells from six independent cord blood (CB) samples. *95% confidence limits, the range within which 95% of the results will typically fall. ND: not done

Figure 1. Comparison of CD34⁺ Cell Expansion in Different StemSpan™ Media Containing CD34⁺ Expansion Supplement

Average expansion of (A) total nucleated cells (TNC), (B) CD34⁺ cells and (C) colony-forming units (CFU), normalized relative to the values obtained in StemSpan™ SFEM (grey bars) after culturing purified hematopoietic CD34⁺ cord blood cells (n=6) for 7 days in StemSpan™ SFEM, SFEM II (blue bars) or AOF (orange bars) media containing CD34⁺ Expansion Supplement. Vertical lines indicate 95% confidence limits, the range within which 95% of results will typically fall. Cell yields in StemSpan™ SFEM II were on average ~60% higher than in StemSpan™ SFEM and StemSpan™-AOF. *p<0.001, #p<0.05 (paired t-test, n=6 in A and B, n=4 in C).

Note: Data for StemSpan™-AOF shown were generated with the original phenol red-containing version StemSpan™-ACF (Catalog #09855). However internal testing showed that the performance of the new phenol red-free, cGMP-manufactured version, StemSpan™-AOF (Catalog #100-0130) was comparable.