Session 3: Expansion of Human Airway Epithelial Cells

Learning Objectives

After this session, you will be able to:

Perform the expansion steps for culturing human airway epithelial cells (HAECs) in PneumaCult™-Ex Plus Medium
Assess culture morphology of human bronchial epithelial cells (HBECs) during the expansion phase

Estimated time for completion: 10 minutes

Note: In this session, we use HBECs as an example. The procedural steps are also applicable for culturing human small airway epithelial cells (HSAECs) using PneumaCult™-ALI-S Medium. For more details, refer to the Product Information Sheet (PIS) for PneumaCult™-ALI-S Medium.

Video: Expansion of HBECs

Supplementary Resources

Poster PneumaCult™-Ex Plus, a Novel Defined and Feeder-Free Medium, Supports the Improved Expansion of Primary Human Airway Epithelial Cells and Maintenance of their Mucociliary Differentiation Potential in Later Passages
Brochure PneumaCult™ Culture Media for Human Airway Epithelial Cells

Test Your Knowledge

See how much you learned from Session 3 before proceeding to Session 4.