Human Peripheral Blood-Derived M1 Macrophages, SF-Cultured, Frozen
Primary human cells, frozen
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Overview
Peripheral blood (PB) monocytes are isolated by negative immunomagnetic selection and cultured for 5 days in serum-free ImmunoCult™-SF Macrophage Medium (Catalog #10961) with macrophage colony-stimulating factor (M-CSF), including 2 days of activation with lipopolysaccharide (LPS) and interferon-gamma (IFN-γ) to generate M1 macrophages.
Cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.
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Protocols and Documentation
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
