Expansion of CD34+ Cells in Serum-Free Culture

StemSpan™ serum-free expansion media is recommended for the expansion and culture of hematopoietic cells, when supplemented with appropriate cytokines and supplements. We offer a number of StemSpan™ Cytokine Cocktails (CC) and Supplements optimized for the expansion of human CD34+ progenitors cells in serum-free culture. To help you select the appropriate StemSpan™ cytokine supplement for your application, we’ve outlined some of the features and differences between these products below:

PRODUCT CATALOG #
(SIZE) 		COMPONENTS

 FEATURES PERFORMANCE*
(in 7 day cultures)
StemSpan™ CC100

 02690

(1 mL,

100X)

  • rh Flt3L
  • rh SCF
  • rh IL-3
  • rh IL-6
  • Contains both early- and late-acting cytokines
  • Stimulates rapid and strong proliferation of human hematopoietic cells, including CD34+ progenitors
  • Generates large numbers of hematopoietic cells in culture, including CD34+ cells and more mature cells.
  • Preferable over CC110 if high cell yields are important
    		•
  • TNC fold expansion:

    • ~20-60-fold

  • CD34+ cells fold expansion:

    • at most ~10-fold
    StemSpan™ CC110

    		02697

    (1 mL,

    100X)

    • rh Flt3L
    • rh SCF
    • rh TPO
  • Contains early-acting cytokines
  • Stimulates proliferation of human hematopoietic cells, including CD34+ progenitors
  • Generates similar expansion of CD34+ cells, but lower total nucleated cell (TNC) yields and higher CD34+ cell frequencies than CC100
  • Preferable over CC100 if high CD34+ cell purity is important
  • Used primarily to activate stem and immature progenitor cell cycling in short term cultures (i.e. for lentiviral or retroviral gene transfer procedures aimed at genetic modification of stem/progenitor cells).
    		•
    StemSpan™ CD34+ Expansion Supplement 02691

    (10 mL,

    10X)
    • rh Flt3L
    • rh SCF
    • rh IL-3
    • rh IL-6
    • rh TPO
    • other additives
  • Stimulates selective expansion of human CD34+ hematopoietic stem and progenitor cells (HSPCs)
  • Generates similar overall expansion of TNCs, but much higher expansion of CD34+ cells compared to CC100 and CC110
  • Preferable over CC100 and CC110 if high CD34+ cell yields and purity are important.
  • Used to achieve high cell yields and also maintain high CD34 expression in cultures.
    		•
  • TNC fold expansion:

    • ~20-60-fold

  • CD34+ cells fold expansion:

    • >10 to 40-fold
    *NOTE: The magnitude of expansion of hematopoietic cells and progenitors is variable between donors and is also dependent on a number of factors including: the cell source, input cell population, culture medium, cell density, time in culture and refeeding or passaging procedures.

    These StemSpan™ Cytokine Cocktails and Supplements are intended for use in combination with our StemSpan™ serum-free expansion media, including StemSpan™ SFEM, SFEM II and ACF.

    For further information on our StemSpan™ products, please contact techsupport@stemcell.com.