EC-Cult™-XF Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate (Component #07130; included in EC-Cult™-XF Culture Kit). For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required. EC-Cult™-XF Medium must be supplemented with Heparin Solution (Catalog #07980). Since Heparin Solution contains non-human animal-derived components, the complete medium will not be xeno-free.
• Serum-free formulation supports the derivation, expansion, and maintenance of ECFCs
• Rigorous raw material screening and quality control minimize lot-to-lot variability and increase reproducibility between experiments
- EC-Cult™-XF Basal Medium, 150 mL
- EC-Cult™-XF 2.5X Supplement, 100 mL
- Animal Component-Free Cell Attachment Substrate, 1 mL
Data and Publications
Figure 1. EC-Cult™ Medium Enables Long-Term Expansion of HUVECs in an FBS-Free Setting
Cryopreserved human umbilical vein endothelial cells (HUVECs) were thawed in either EC-CultTM Medium or a fetal bovine serum (FBS)-containing commercial medium at 1x10^4 cells/well (6-well plate) and passaged in the same media. Cells cultured in EC-Cult™ Medium were passaged using the Animal Component-Free Cell Dissociation Kit. Cells cultured in FBS-containing commercial medium were cultured and passaged according to manufacturer’s protocol. HUVECs cultured in EC-Cult™ Medium display the typical cobblestone morphology of endothelial cells.
Figure 2. HUVECs Cultured Long-Term in EC-Cult™ Medium Maintain High Levels of Endothelial Markers
Human umbilical vein endothelial cells (HUVECs) cultured and passaged in EC-Cult™ Medium (analyzed by flow cytometry) retained the endothelial markers CD31 and CD144 through 19 passages.
Figure 3. HUVECs Form Tubular Networks When Cultured in EC-Cult™ Medium
Human umbilical vein endothelial cells (HUVECS) were expanded for 18 passages in EC-Cult™ Media or a commercial FBS-containing medium. The cells were then plated at 1.0 x 10^4 cells/well (96-well plate) in EC-Cult™ Medium and grown for 24 hours on Corning® Matrigel®. Network formations were imaged at different time points after plating. HUVECS cultured in EC-Cult™ media formed higher quality, more defined tubular branching points.
Figure 4. EC-Cult™ Medium Can Be Used to Expand Different Endothelial Cell Types
Cryopreserved human umbilical vein endothelial cells (HUVECs), human aortic endothelial cells (HAECs), and dermal human microvascular endothelial cells (dHMVECs) were expanded using EC-Cult™ Medium or other commercial media according to their respective manufacturers’ protocols and counted after each passage. Cells expanded more rapidly and for longer passage numbers in EC-Cult™ Medium.