EasySep™ Mouse F4/80 Positive Selection Kit

Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage

EasySep™ Mouse F4/80 Positive Selection Kit

Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage

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Immunomagnetic positive selection of F4/80+ cells from mouse splenocytes, lung tissue, and peritoneal lavage
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Product Advantages


  • Fast and easy-to-use

  • Up to 90% purity

  • No columns required

  • Isolated cells are not fluorochrome-labeled

What's Included

  • EasySep™ Mouse F4/80 Positive Selection Component A, 0.3 mL
  • EasySep™ Mouse F4/80 Positive Selection Component B, 0.6 mL
  • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
  • Normal Rat Serum, 2 mL

Overview

Easily isolate F4/80+ macrophages in as little as 24 minutes—without the use of columns—from mouse splenocytes, lung tissue, and peritoneal lavage.

Using immunomagnetic positive selection, F4/80+ cells are labeled with antibodies and dextran-coated magnetic particles (RapidSpheres™), for column-free cell separation using an EasySep™ magnet. Isolated cells are highly purified and ready for your downstream applications.

Visit www.EasySep.com to learn more about column-free EasySep™ cell separation technology.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Macrophages
Species
Mouse
Sample Source
Lung, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology, Infectious Diseases

Data Figures

FACS plot showing cell populations from lung tissue pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 1. Typical Profile for F4/80+ Cells Isolated from Lung Tissue Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.

FACS plot showing cell populations from peritoneal lavage pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 2. Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 94.4 ± 2.9% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 40.9% and 94.1%, respectively.

FACS plot showing cell populations from spleen pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 3. Typical Profile for F4/80+ Cells Isolated from Spleen Using the EasySep™ Mouse F4/80 Positive Selection Kit

Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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100-0659
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English
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Safety Data Sheet 1
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100-0659
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English
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100-0659
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Safety Data Sheet 4
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100-0659
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English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.