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EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

From: 797 USD
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Immunomagnetic positive selection cell isolation kit
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Product Advantages


  • Fast and easy-to-use

  • No columns required

What's Included

  • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887RF)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit is designed to isolate CD138+ (syndecan-1) cells from whole blood and bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).

This product replaces the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #18387) for even faster cell isolations.

Take our free On-Demand CD138+ Cell Isolation Course to learn how to process bone marrow samples and isolate CD138+ plasma cells to increase sensitivity in FISH testing for multiple myeloma. You can also browse our Frequently Asked Questions (FAQs) about isolating CD138+ plasma cells to improve FISH sensitivity.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Myeloma, Plasma
Species
Human
Sample Source
Bone Marrow, Whole Blood
Selection Method
Positive
Application
ELISA
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Starting with fresh whole blood spiked with a multiple myeloma cell line, U266, the CD138+ cell content of the selected fraction typically ranges from 83.7 - 98.3%. In the above example, the purities of the start and final isolated fractions are 9.1% and 90.4%, respectively. NOTE: For samples with CD138+ starting frequency < 10 - 15%, the CD138+ purity of the isolated fraction may be variable. NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887
Lot #
All
Language
Multi
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887RF
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887RF
Lot #
All
Language
Multi
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II
Catalog #
17887RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human B Cell Isolation Product Selection
Brochure
Human B Cell Isolation Product Selection
HLA Antibodies for Purity Assessment
Brochure
HLA Antibodies for Purity Assessment
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Enhance Sensitivity of Multiple Myeloma Testing with Purified CD138+ Plasma Cells
Technical Bulletin
Enhance Sensitivity of Multiple Myeloma Testing with Purified CD138+ Plasma Cells
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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