EasySep™ Human Eosinophil Isolation Kit

11-Minute cell isolation kit using immunomagnetic negative selection

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From: 813 USD

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11-Minute cell isolation kit using immunomagnetic negative selection
From: 813 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Eosinophil Isolation Kit is designed to isolate eosinophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-eosinophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

The kit is compatible with cells prepared using either Lymphoprep™ (Catalog #07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).

This product replaces the EasySep™ Human Eosinophil Enrichment Kit (Catalog #19256) for even faster cell isolations.
Advantages:
• Fast, easy-to-use and column-free
• Up to 99% purity
• Untouched, viable cells
Components:
  • EasySep™ Human Eosinophil Isolation Kit (Catalog #17956)
    • EasySep™ Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • RoboSep™ Human Eosinophil Isolation Kit with Filter Tips (Catalog #17956RF)
    • EasySep™ Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets
Species:
Human
Sample Source:
PMNC; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(8)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
2:10
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Figure 1. Typical EasySep™ Human Eosinophil Isolation Profile

Starting with whole blood prepared using RBC lysis or HetaSep™, the eosinophil content (CD16-CD66b+CD45+) of the isolated fraction is typically 96.5 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above examples, the purities of the start and final isolated fractions prepared using RBC lysis are 1.8% and 97.8% (gated on CD45+), respectively, and when prepared using HetaSep™, the purities are 2.4% and 96.8% (gated on CD45+), respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.