EasySep™ Human Basophil Isolation Kit

13-Minute cell isolation kit using immunomagnetic negative selection

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human Basophil Isolation Kit

13-Minute cell isolation kit using immunomagnetic negative selection

From: 823 USD
Catalog #
17969_C
13-Minute cell isolation kit using immunomagnetic negative selection

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 98% purity

  • Isolated cells are untouched

What's Included

  • EasySep™ Human Basophil Isolation Kit (Catalog #17969)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
  • RoboSep™ Human Basophil Isolation Kit with Filter Tips (Catalog #17969RF)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.

This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Figure 1. Typical EasySep™ Human Basophil Isolation Profile

Starting with HetaSep™-processsed human peripheral blood, the basophil content (CD45+CD123+IgE+) of the isolated fraction is typically 94.0 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.86% and 95.6% (gated on CD45+), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17969
Lot #
All
Language
English
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17969RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

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