Anti-Phospho-SHIP Antibody, Polyclonal

Rabbit polyclonal antibody against human, mouse phosphoSHIP

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Rabbit polyclonal antibody against human, mouse phosphoSHIP
482 USD

Overview Related Products Scientific Resources Data and Publications

Overview

Phospho-SHIP is a highly phosphorylated form of SHIP. SH2-containing inositol phosphatase (SHIP) is a 145 kDa hematopoietic-restricted protein that becomes tyrosine-phosphorylated and associated with the adaptor protein, Shc, following cytokine, growth factor, chemokine, and immunoreceptor stimulation. SHIP also hydrolyzes the critical phosphatidylinositol (PI)-3-kinase (PI3K)-generated second messenger, PI-3,4,5-P3 (PIP3), to PI-3,4-P2 1,2 and therefore acts as an important negative regulator of the PI3K pathway. This antibody reacts with mouse and human tyrosine-phosphorylated SHIP.
Subtype:
Primary Antibodies
Target Antigen:
PhosphoSHIP
Alternative Names:
p150Ship; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; SHIP-1; SHIP1; SIP-145; s-SHIP
Reactive Species:
Mouse; Human
Conjugation:
Unconjugated
Host Species:
Rabbit
Cell Type:
Granulocytes and Subsets; Lymphocytes; Myeloid Cells
Application:
Immunoblotting; Immunoprecipitation; Flow Cytometry; Immunofluorescence
Area of Interest:
Immunology
Clone:
Not applicable
Gene ID:
3635 (human), 16331 (mouse)
Isotype:
Not applicable

Scientific Resources

Product Documentation

Educational Materials

(2)
Brochure
Complete Antibody Listing
Wallchart
Human Immune Cytokines

Data and Publications

Data

(A) Flow cytometry analysis of bone marrow derived mast cells (BMMCs) from normal (+/+) or SHIP knockout (-/-) mice. Cells were stimulated with stem cell factor (SCF; filled histogram) or left unstimulated (solid line historgram), and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(B) BMMCs were either stimulated with SCF or left unstimulated, and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(C) Western blot analysis of total cell lysates from human TF-1 cells stimulated with IL-3, mouse WEHI-231 B cells stimulated with anti-IgM, and mouse BMMCs stimulated with SCF, and probed with Anti-Phospho-SHIP Antibody, Polyclonal. The blot was re-probed with an anti-SHIP antibody to show equal loading of unstimulated (C) and stimulated (S) samples. Phospho-SHIP has a predicted molecular mass of 145 kDa.

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