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Preparation of Single-Cell Suspensions

Sample Preparation and Storage

Preparation of Single-Cell Suspensions

Starting with a single-cell suspension is essential for successful downstream cell isolation. Explore these technical resources to help you prepare single-cell suspensions from tissue or cell culture samples. You’ll find protocols and support on topics such as how to prepare a single-cell suspension from mouse spleen and how to treat clumpy cell suspensions.

Learn about the preparation of tissue samples for cell separation >


Tech Tip Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures
Tech Tip Isolating Cells from Atypical Samples
Tech Tip Tissue Dissociation and Digestion Protocols
Protocol How to Reduce Cell Clumping in Single-Cell Suspensions Using DNase I
Protocol How to Prepare a Single-Cell Suspension from Mouse Spleen
Protocol How to Isolate and Stain Mouse Bone Marrow Cells for Flow Cytometry
Protocol Enzymatic Dissociation of Adult Mouse or Rat CNS Tissue into Single-Cell Suspension
Protocol How to Dissociate 3D Neural Organoids into a Single-Cell Suspension
Video How to Prepare a Single-Cell Suspension from Mouse Spleen
Video How to Prepare a Single-Cell Suspension from Mouse Brain Tissue
Video How to Prepare a Single-Cell Suspension from Mouse Lung Tissue
Protocol Dissociation of Mouse Lung Tissue into a Single-Cell Suspension
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Product Information Sheets

For detailed protocols developed specifically for our products, please consult the Product Information Sheet or enter the product keyword or catalog number below.


Learn more about Cell Separation
Methods Library

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