Resazurin (Sodium Salt)
Cell proliferation and viability dye
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Resazurin (Sodium Salt) -
Label for Resazurin (Sodium Salt)
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Overview
Resazurin (7-hydroxy-10-oxidophenoxazin-10-ium-3-one, sodium) is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast or mammalian cells. The blue form of the dye is irreversibly reduced by enzymes in viable cells to generate a highly red-fluorescent product, resorufin, which exhibits an emission maximum at ~595 nm and can be detected by flow cytometry, fluorescence microscopy, and high-throughput screening methods. Resazurin is minimally toxic to living cells, making it suitable for use in long-term cell culture. The dye has also been used to assay L-glutamate and to measure the metabolic activity of mitochondria.
Application:
Flow Cytometry
Area of Interest:
Immunology; Neuroscience; Stem Cell Biology
Chemical Formula:
C₁₂H₆NO₄ · Na
Molecular Weight:
251.2 g/mol
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Figure 1. Resazurin (Sodium Salt)
Chemical structure of Resazurin (Sodium Salt).
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.