Human Peripheral Blood B Cells, Frozen

Primary human cells, frozen
Catalog #
70023_C
Primary human cells, frozen
From: 1,006 USD

Overview

Human primary B cells derived from peripheral blood (PB) are isolated from mononuclear cells (MNCs) using the negative immunomagnetic selection technique. Cells are untouched and ready for downstream applications. PB was collected using acid-citrate-dextrose solution A (ACDA) as the anticoagulant.

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

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Contains
• CryoStor® CS10
Subtype
Frozen
Cell Type
B Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Donor Status
Normal
Purity
The purity of B cells is ≥ 85% by flow cytometry.

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Peripheral Blood B Cells, Frozen
Catalog #
70023, 70023.1
Lot #
All
Language
English

Educational Materials (4)

Brochure
Tools For Your Immunology Research
Brochure
Human Primary Cells
Video
How to Thaw Frozen Human Primary Cells
6:05
How to Thaw Frozen Human Primary Cells
Webinar
Online Immunology Journal Club: The Potential of Antibody-Producing B Cells for Modelling and Therapy
34:58
Online Immunology Journal Club: The Potential of Antibody-Producing B Cells for Modelling and Therapy

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Cryopreserved B Cells Show High Viability and Produce IgG Antibodies Upon Stimulation

(A) B Cells (Catalog #70023) cryopreserved in CryoStor® CS10 (Catalog #07930) show high viability upon thawing (average = 96.3 ± 0.6 %, n = 6). (B) B cells were cultured for 1 week in RPMI 1640 Medium (Catalog #36750) with 10% FBS, 2mM L-Glutamine (Catalog #07100), 10mM HEPES (Catalog #07200) and 55 µM β-mercaptoethanol and either left unstimulated (control) or stimulated with CD40 in the presence of IL-21 (activated). Activated B cells produce significantly more IgG antibodies compared to unstimulated controls as measured by ELISA.

Publications (1)

Journal of medicinal chemistry 2014 MAY Discovery of GS-9973, a selective and orally efficacious inhibitor of spleen tyrosine kinase. Currie KS et al.

Abstract

Spleen tyrosine kinase (Syk) is an attractive drug target in autoimmune, inflammatory, and oncology disease indications. The most advanced Syk inhibitor, R406, 1 (or its prodrug form fostamatinib, 2), has shown efficacy in multiple therapeutic indications, but its clinical progress has been hampered by dose-limiting adverse effects that have been attributed, at least in part, to the off-target activities of 1. It is expected that a more selective Syk inhibitor would provide a greater therapeutic window. Herein we report the discovery and optimization of a novel series of imidazo[1,2-a]pyrazine Syk inhibitors. This work culminated in the identification of GS-9973, 68, a highly selective and orally efficacious Syk inhibitor which is currently undergoing clinical evaluation for autoimmune and oncology indications.
View All Publications

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