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EasySep™ Release Human PE Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

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EasySep™ Release Human PE Positive Selection Kit

Immunomagnetic positive selection of particle-free human cells labeled with PE-conjugated antibodies

1 x 109 cells
Catalog #17654
710 USD

Required Products

Overview

The EasySep™ Release Human PE Positive Selection Kit is designed to isolate cells that are labeled with PE-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, PE-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

This product replaces the EasySep™ Human PE Positive Selection Kit (Catalog #18551), providing highly purified particle-free cells.
Advantages:
• Highly purified cells labeled with PE-conjugated antibodies isolated from human or mouse tissues in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components:
  • EasySep™ Release Human PE Positive Selection Kit (Catalog #17654)
    • EasySep™ Release PE Positive Selection Cocktail, 0.5 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer, 1 mL
    • Anti-Human CD32 Blocker, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Mesenchymal Stem and Progenitor Cells; Monocytes; Myeloid Cells; NK Cells; Other; Plasma Cells; T Cells; Marrow Stromal Cells; Mononuclear Cells; Macrophages
Species:
Human
Sample Source:
Other; PBMC; Leukapheresis
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(8)

Data and Publications

Data

Starting with fresh PBMCs, the purities of the start and final isolated fractions are 46.9% and 98.8%, respectively, using a PE-conjugated anti-human CD45RO antibody and EasySep™ Release Human Positive Selection Kit.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.