EasySep™ Mouse Epithelial Cell Enrichment Kit II

Immunomagnetic negative selection kit

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EasySep™ Mouse Epithelial Cell Enrichment Kit II

Immunomagnetic isolation of untouched mouse epithelial cells

1 x 109 cells
Catalog #19868
607 USD

Required Products

Overview

The EasySep™ Mouse Epithelial Cell Enrichment Kit II is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted non-epithelial cells with biotinylated antibodies and magnetic particles. The magnetically labeled cells are separated from untouched desired cells by using an EasySep™ magnet and simply pouring the desired cells into a new tube.

This product replaces the EasySep™ Epithelial Cell Enrichment Kit (Catalog #19758) for even faster isolations. It also replaces the cell enrichment portion of the EasySep™ Mouse Mammary Stem Cell Enrichment Kit (Catalog #19757). Additionally, Anti-Mouse CD24 Antibody, Clone M1/69, PE (Catalog #60099PE) and Anti-Mouse CD49f Antibody, Clone GoH3, FITC (Catalog #60037FI) are required to complete the kit.
Advantages:
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components:
  • EasySep™ Mouse Epithelial Cell Enrichment Kit II (Catalog #19868)
    • EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
    • EasySep™ Biotin Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype:
Cell Isolation Kits
Cell Type:
Mammary Cells; Prostate Cells
Species:
Mouse
Sample Source:
Other; Primary
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Epithelial Cell Biology

Scientific Resources

Product Documentation

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Educational Materials

(4)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with mouse mammary tissues, the epithelial cell content of the enriched fraction is typically 96.97 ± 0.54% (mean ± SD using the purple EasySep™ Magnet). In the above example, the percentages of epithelial cells in the start and final enriched fractions are 19.9% and 97.4%, respectively.

Publications

(1)
Nature communications 2019

Sterol regulatory element binding protein 1 couples mechanical cues and lipid metabolism.

R. Bertolio et al.

Abstract

Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that regulate lipid biosynthesis and adipogenesis by controlling the expression of several enzymes required for cholesterol, fatty acid, triacylglycerol and phospholipid synthesis. In vertebrates, SREBP activation is mainly controlled by a complex and well-characterized feedback mechanism mediated by cholesterol, a crucial bio-product of the SREBP-activated mevalonate pathway. In this work, we identified acto-myosin contractility and mechanical forces imposed by the extracellular matrix (ECM) as SREBP1 regulators. SREBP1 control by mechanical cues depends on geranylgeranyl pyrophosphate, another key bio-product of the mevalonate pathway, and impacts on stem cell fate in mouse and on fat storage in Drosophila. Mechanistically, we show that activation of AMP-activated protein kinase (AMPK) by ECM stiffening and geranylgeranylated RhoA-dependent acto-myosin contraction inhibits SREBP1 activation. Our results unveil an unpredicted and evolutionary conserved role of SREBP1 in rewiring cell metabolism in response to mechanical cues.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.