EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
Catalog #
17881_C
Immunomagnetic positive selection cell isolation kit
From: 683 USD
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Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. "The Big Easy" EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit is designed to isolate highly purified CD15+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD15 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD15 antigen is expressed on granulocytes and monocytes.

This product replaces the EasySep™ Human Whole Blood CD15 Positive Selection Kit (Catalog #18681) and EasySep™ HLA Whole Blood CD15 Positive Selection Kit (Catalog #18681HLA), for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit (Catalog #17881)
    • EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit with Filter Tips (Catalog #17881RF)
    • EasySep™ Human Whole Blood CD15 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Myeloid Cells
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English

Educational Materials (10)

Brochure
Cell Separation Products for HLA Analysis
Brochure
EasySep™ Cell Separation Technology
Brochure
HLA Antibodies for Purity Assessment
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with human whole blood, the CD15+ cell content of the isolated fraction is typically 99.2 ± 1.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 57.6% and 99.9%, respectively.

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