Caspase-3/7 Activity Flow Cytometry Kit, Green

For detection of active caspase-3 in mammalian cells undergoing apoptosis

Caspase-3/7 Activity Flow Cytometry Kit, Green

For detection of active caspase-3 in mammalian cells undergoing apoptosis

From: 599 USD
Catalog #
100-0923_C
For detection of active caspase-3 in mammalian cells undergoing apoptosis

Product Advantages

⦁ TF2-DEVD-FMK detects caspase-3/7 activity
⦁ Excitation wavelength: 503 nm
⦁ Emission wavelength: 525 nm
⦁ Extinction coefficient: 75,000 cm⁻¹M⁻¹
⦁ Easily scalable for high-throughput assays
⦁ Verified for use with flow cytometry

What's Included

  • Caspase-3/7 Activity Flow Cytometry Kit, Green (Catalog #100-0923)
    • TF2-DEVD-FMK (500X), 100 µL
    • Propidium Iodide (500x), 100 µL
    • Assay Buffer, 50 mL

Overview

Efficiently detect active caspase-3 in mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Flow Cytometry Kit, Green. This reagent kit uses a quick and simple staining protocol that is highly sensitive and compatible with standard flow cytometers.

Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit includes TF2-DEVD-FMK—a cell-permeable and non-toxic fluorescent substrate—as well as an assay buffer and propidium iodide. When added to apoptotic cells, TF2-DEVD-FMK irreversibly binds to activated caspase-3, resulting in the retention of the green fluorescent reagent within cells that can be detected by flow cytometry.

Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases—caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). Caspase-3/7 Activity Flow Cytometry Kit, Green is useful for screening caspase-3 inhibitors and quantifying apoptotic cells containing active caspase-3.
Application
Flow Cytometry
Area of Interest
Cancer
CAS Number
67-68-5, 25535-16-4

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0923
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0923
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0923
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0923
Lot #
All
Language
English

Data and Publications

Figure 1. Caspase-3/7 Activity Flow Cytometry Kit, Green

Jurkat cells were treated with 0.2% DMSO (Control) or 20 µM camptothecin (CPT), and in the presence or absence of Z-VAD-FMK (a pan-caspase inhibitor). Active caspase-3/7 was detected by flow cytometry, using the Caspase-3/7 Activity Flow Cytometry Kit, Green. A representative plot of three independent experiments is depicted in the figure above

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