Caspase-3/7 Activity Flow Cytometer Kit, Green

For detection of active caspase-3 in mammalian cells undergoing apoptosis

Caspase-3/7 Activity Flow Cytometer Kit, Green

For detection of active caspase-3 in mammalian cells undergoing apoptosis

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For detection of active caspase-3 in mammalian cells undergoing apoptosis
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Product Advantages


  • TF2-DEVD-FMK detects caspase-3/7 activity

  • Excitation wavelength: 503 nm

  • Emission wavelength: 525 nm

  • Extinction coefficient: 75,000 cm⁻¹M⁻¹

  • Easily scalable for high-throughput assays

  • Verified for use with flow cytometry

What's Included

  • Caspase-3/7 Activity Flow Cytometry Kit, Green (Catalog #100-0923)
    • TF2-DEVD-FMK (500X), 100 µL
    • Propidium Iodide (500x), 100 µL
    • Assay Buffer, 50 mL

Overview

Efficiently detect active caspase-3 in mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Flow Cytometry Kit, Green. This reagent kit uses a quick and simple staining protocol that is highly sensitive and compatible with standard flow cytometers.

Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit includes TF2-DEVD-FMK—a cell-permeable and non-toxic fluorescent substrate—as well as an assay buffer and propidium iodide. When added to apoptotic cells, TF2-DEVD-FMK irreversibly binds to activated caspase-3, resulting in the retention of the green fluorescent reagent within cells that can be detected by flow cytometry.

Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases—caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). Caspase-3/7 Activity Flow Cytometry Kit, Green is useful for screening caspase-3 inhibitors and quantifying apoptotic cells containing active caspase-3.
Application
Flow Cytometry
Area of Interest
Cancer
CAS Number
67-68-5, 25535-16-4

Data Figures

Figure 1. Caspase-3/7 Activity Flow Cytometry Kit, Green

Jurkat cells were treated with 0.2% DMSO (Control) or 20 µM camptothecin (CPT), and in the presence or absence of Z-VAD-FMK (a pan-caspase inhibitor). Active caspase-3/7 was detected by flow cytometry, using the Caspase-3/7 Activity Flow Cytometry Kit, Green. A representative plot of three independent experiments is depicted in the figure above

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Catalog #
100-0923
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All
Language
English
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Safety Data Sheet 1
Catalog #
100-0923
Lot #
All
Language
English
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Safety Data Sheet 2
Catalog #
100-0923
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All
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English
Document Type
Safety Data Sheet 3
Catalog #
100-0923
Lot #
All
Language
English