Anti-Mouse IFN-gamma Antibody, Clone XMG1.2

Rat monoclonal IgG1 antibody against mouse IFN-gamma

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Rat monoclonal IgG1 antibody against mouse IFN-gamma
From: 59 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The XMG1.2 antibody reacts with mouse interferon-gamma (IFN-γ), a glycosylated type II interferon comprising a homodimer of ~20 kDa subunits in its active form. IFN-γ is produced primarily by activated T lymphocytes and natural killer (NK) cells in response to antigens, including alloantigens, mitogens, and enterotoxins. IFN-γ binds with high affinity to the α subunit of the IFN-γ receptor, resulting in the ligand-induced assembly of a tetrameric α2β2 receptor complex capable of mediating the cellular response to the cytokine through activation of the JAK-STAT pathway. The IFN-γ receptor is expressed on the surface of all nucleated cells. IFN-γ has important functions in a wide range of biological processes, including macrophage activation, mediating the immune response to viruses and bacteria, and modulating cellular proliferation and apoptosis. The XMG1.2 antibody is suitable for detection of intracellular IFN-γ by methods such as flow cytometry, when paired with an appropriate secondary antibody. The XMG1.2 antibody is also widely used in various assays to neutralize the functional activity of IFN-γ.
Subtype:
Primary Antibodies
Target Antigen:
IFNγ
Alternative Names:
IFN-γ, Immune interferon, Interferon-gamma, Macrophage-activating factor (MAF), T cell interferon, Type II interferon
Reactive Species:
Mouse
Conjugation:
APC; Biotin; FITC; PE
Host Species:
Rat
Cell Type:
Dendritic Cells; Macrophages; NK Cells; T Cells
Application:
ELISA; ELISpot; Flow Cytometry
Area of Interest:
Immunology
Clone:
XMG1.2
Gene ID:
15978
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(3)
Brochure
Complete Antibody Listing
Brochure
Tools For Your Immunology Research
Brochure
Immunology Antibodies

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization

Data and Publications

Data

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.

Figure 1. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC

Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, Biotin.

Figure 2. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, Biotin

Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-γ Antibody, Clone XMG1.2, Biotin, followed by streptavidin (SAV) PE.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody, Clone XMG1.2, FITC.

Figure 3. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC

Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.

Figure 4. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE

Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.