Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2

Rat monoclonal IgG2b antibody against mouse CD16/CD32
Catalog #
60161_C
Rat monoclonal IgG2b antibody against mouse CD16/CD32
From: 54 USD

Overview

The 2.4G2 antibody reacts with a shared epitope of Fc-gamma receptors CD16 (FcRIII) and CD32 (FcRII) in the mouse. CD16 and CD32 are low affinity receptors for the Fc portion of IgG, with CD32 having a higher affinity for IgG aggregates and immune complexes. CD16 and CD32 are expressed on B cells, monocytes, macrophages, NK cells, neutrophils, granulocytes, mast cells, and dendritic cells. The binding of immunoglobulins to Fc receptors is involved in multiple immunological functions, including phagocytosis, cytotoxocity, and the modulation of immune responses. The 2.4G2 antibody blocks the interaction between IgG and CD16 or CD32, and therefore is used as a pre-treatment to minimize non-specific binding of antibodies to FcR-bearing cells in applications such as flow cytometry, cell sorting, and immunofluorescence.
Subtype
Primary Antibodies
Target Antigen
CD16/CD32
Alternative Names
FCGR2, FCGR3, FC Receptor, IGFR2, IGFR3, Ly-17
Reactive Species
Mouse
Conjugation
FITC, Unconjugated
Host Species
Rat
Cell Type
Dendritic Cells, Granulocytes and Subsets, Macrophages, Monocytes, Myeloid Cells, NK Cells, T Cells, T Cells, Other Subsets
Application
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Area of Interest
Immunology
Clone
2.4G2
Gene ID
14130/14131
Isotype
IgG2b, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2
Catalog #
60161, 60161.1, 60161.2
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, FITC
Catalog #
60161FI, 60161FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2
Catalog #
60161, 60161.1, 60161.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, FITC
Catalog #
60161FI, 60161FI.1
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Data and Publications

Data

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI; solid line histogram).

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