Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2

Rat monoclonal IgG2b antibody against mouse CD16/CD32

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Rat monoclonal IgG2b antibody against mouse CD16/CD32
From: 48 USD

Overview

The 2.4G2 antibody reacts with a shared epitope of Fc-gamma receptors CD16 (FcRIII) and CD32 (FcRII) in the mouse. CD16 and CD32 are low affinity receptors for the Fc portion of IgG, with CD32 having a higher affinity for IgG aggregates and immune complexes. CD16 and CD32 are expressed on B cells, monocytes, macrophages, NK cells, neutrophils, granulocytes, mast cells, and dendritic cells. The binding of immunoglobulins to Fc receptors is involved in multiple immunological functions, including phagocytosis, cytotoxocity, and the modulation of immune responses. The 2.4G2 antibody blocks the interaction between IgG and CD16 or CD32, and therefore is used as a pre-treatment to minimize non-specific binding of antibodies to FcR-bearing cells in applications such as flow cytometry, cell sorting, and immunofluorescence.
Subtype:
Primary Antibodies
Target Antigen:
CD16/CD32
Alternative Names:
FCGR2, FCGR3, FC Receptor, IGFR2, IGFR3, Ly-17
Reactive Species:
Mouse
Conjugation:
FITC; Unconjugated
Host Species:
Rat
Cell Type:
Dendritic Cells; Granulocytes and Subsets; Monocytes; Myeloid Cells; NK Cells; T Cells; T Cells, Other Subsets; Macrophages
Application:
Flow Cytometry; Immunofluorescence; Immunoprecipitation; Functional Assay
Area of Interest:
Immunology
Clone:
2.4G2
Gene ID:
14130/14131
Isotype:
IgG2b, kappa

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Data

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI; solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.