Anti-Human CD83 Antibody, Clone HB15e
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83
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Overview
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).
Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 (solid line histogram).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).
Figure 6. Data for PE-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
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Anti-Human CD83 Antibody, Clone HB15e
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.