Anti-Human CD83 Antibody, Clone HB15e

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83

Anti-Human CD83 Antibody, Clone HB15e

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83

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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83
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Overview

The HB15e antibody reacts with CD83, an ~45 kDa type I transmembrane glycoprotein and member of the Siglec protein family, containing an extracellular V-type Ig-like domain and a C-terminal cytoplasmic tail. A soluble form of CD83 has also been identified. CD83 is expressed predominantly on circulating and tissue dendritic cells, including Langerhan cells and interdigitating reticulum cells. It is expressed weakly on activated lymphocytes and may also be found on neutrophils, monocytes and macrophages. Expression of CD83 is typically transient, except on dendritic cells, and correlates with upregulation of HLA class II antigen. Its function, though ill-defined, is essential for the development and maintenance of the immune system and evidence suggests it may be involved in cell adhesion and signaling, antigen presentation, and lymphocyte activation. The soluble form of CD83 appears to be immunosuppressive. The epitope on CD83 bound by the HB15e antibody reportedly overlaps with that of antibody 1E11.
Subtype
Primary Antibodies
Target Antigen
CD83
Alternative Names
B-cell activation protein, BL11, HB15
Reactive Species
Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Rhesus
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Dendritic Cells
Species
Human, Non-Human Primate
Application
Flow Cytometry, Immunoblotting, Immunohistochemistry
Area of Interest
Immunology
Clone
HB15e
Gene ID
9308
Isotype
IgG1, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60107AD
Lot #
All
Language
English
Catalog #
60107PE.1, 60107PE, 60107PE.2
Lot #
All
Language
English
Catalog #
60107AZ.1, 60107AZ
Lot #
All
Language
English
Catalog #
60107.1, 60107
Lot #
All
Language
English
Catalog #
60107FI, 60107FI.1
Lot #
All
Language
English
Catalog #
60107BT, 60107BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60107AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60107PE.1, 60107PE, 60107PE.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60107AZ.1, 60107AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60107.1, 60107
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60107FI, 60107FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60107BT, 60107BT.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications