Anti-Human CD66b Antibody, Clone G10F5

Mouse monoclonal IgM antibody against human, chimpanzee CD66b
Catalog #
60086_C
Mouse monoclonal IgM antibody against human, chimpanzee CD66b
From: 125 USD

Overview

The G10F5 antibody reacts with CD66b (previously denoted CD67), an ~95 kDa single-chain glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed by neutrophilic and eosinophilic granulocytes. CD66b is a member of the carcinoembryonic antigen (CEA)-like subfamily of proteins within the immunoglobulin superfamily and possesses two Ig-like C2-type domains and one Ig-like V-type domain. In neutrophils, CD66b is localized within granules in the cytoplasm as well as being expressed on the plasma membrane. Surface expression levels increase upon granulocyte activation, an effect induced by stimulators such as phorbol myristate acetate (PMA), calcium ionophore, and N-formylmethionyl-leucyl-phenylalanine (FMLP). CD66b appears to play a role as a signaling receptor involved in cell adhesion, phagocytosis, and chemotaxis by regulating granulocyte activation and adhesion to several proteins, including CD66c, galectin-3, fibronectin, and E-selectin.
Subtype
Primary Antibodies
Target Antigen
CD66b
Alternative Names
Carcinoembryonic antigen-related cell adhesion molecule 8, CD67, CEACAM8, CGM6, NCA-95
Reactive Species
Chimpanzee, Human
Conjugation
Pacific Blue, APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Human, Non-Human Primate
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
G10F5
Gene ID
1088
Isotype
IgM, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5
Catalog #
60086
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, APC
Catalog #
60086AZ, 60086AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, FITC
Catalog #
60086FI, 60086FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, PE
Catalog #
60086PE, 60086PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, Pacific Blue™
Catalog #
60086PB, 60086PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5
Catalog #
60086
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, APC
Catalog #
60086AZ, 60086AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, FITC
Catalog #
60086FI, 60086FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, PE
Catalog #
60086PE, 60086PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD66b Antibody, Clone G10F5, Pacific Blue™
Catalog #
60086PB, 60086PB.1
Lot #
All
Language
English

Educational Materials (4)

Brochure
Reliable Antibodies for Your Research
Brochure
HLA Antibodies for Purity Assessment
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 (Catalog #60069), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, APC (filled histogram) or a mouse IgM, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa APC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of whole blood nucleated cells processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa APC isotype control antibody is shown (solid line histogram).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC (filled histogram) or a mouse IgM, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ Whole Blood CD66b Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa FITC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa FITC isotype control antibody is shown (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (Catalog #60069PE) (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM30, PE is shown (solid line histogram). (C) Flow cytometry analysis of whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti­Human CD66b Antibody, Clone G10F5, PE. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE is shown (solid line histogram).

Figure 5. Data for PB-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD66b Antibody, Clone G10F5, Pacific Blue™ (filled histogram) or a mouse IgM, kappa Pacific Blue™ isotype control antibody (solid line histogram).

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