Anti-Human CD66b Antibody, Clone G10F5
Mouse monoclonal IgM antibody against human, chimpanzee CD66b
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Overview
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 (Catalog #60069), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, APC (filled histogram) or a mouse IgM, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa APC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of whole blood nucleated cells processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa APC isotype control antibody is shown (solid line histogram).
Figure 3. Data for FITC-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC (filled histogram) or a mouse IgM, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ Whole Blood CD66b Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa FITC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgM, kappa FITC isotype control antibody is shown (solid line histogram).
Figure 4. Data for PE-Conjugated
(A) Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD66b Antibody, Clone G10F5, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (Catalog #60069PE) (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD15 Positive Selection Kit and labeled with Anti-Human CD66b Antibody, Clone G10F5, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM30, PE is shown (solid line histogram). (C) Flow cytometry analysis of whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with AntiHuman CD66b Antibody, Clone G10F5, PE. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE is shown (solid line histogram).
Figure 5. Data for PB-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD66b Antibody, Clone G10F5, Pacific Blue™ (filled histogram) or a mouse IgM, kappa Pacific Blue™ isotype control antibody (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
Legal Statement:
Pacific Blue is a trademark of Life Technologies Corporation. Antibodies conjugated to Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
