NeuroCult™ Neuronal Plating Medium

Culture medium for plating dissociated primary tissue-derived neurons for improved survival
NeuroCult™ Neuronal Plating Medium

100 mL
Catalog # 05713
53 USD

Overview

NeuroCult™ Neuronal Plating Medium is a serum-free neuronal basal medium. It is designed for use with BrainPhys™ Neuronal Medium (Catalog #05790) for the plating and culture of primary tissue-derived neurons.
Advantages
• Optimized for the survival of primary tissue-derived neurons
• Tested for compatibility with BrainPhys™ Neuronal Medium
Subtype
Basal Media, Specialized Media
Cell Type
Neurons
Species
Mouse, Rat
Application
Cell Culture, Maintenance
Brand
NeuroCult
Area of Interest
Neuroscience
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
NeuroCult™ Neuronal Plating Medium
Catalog #
05713
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
NeuroCult™ Neuronal Plating Medium
Catalog #
05713
Lot #
All
Language
English

Educational Materials(10)

Brochure
BrainPhys™ Neuronal Medium for Improved Neuronal Function
Brochure
Neural Supplements For High-Quality Neural Cell Cultures
Brochure
Primary Neuronal Culture: Standardized Media and Reagents
Video
0:55
BrainPhys™: A New Way to Culture Neurons
Webinar
27:19
BrainPhys™ Medium Supports the Physiological Activity of Neuronal Tissue in vitro
Webinar
42:39
Standardized Primary Neuronal Culture with NeuroCult™ SM
Webinar
56:27
The Road to Functional Human Neuronal Circuits in Vitro
Scientific Poster
BrainPhys™ Neuronal Medium: A Medium Optimized to Support the Synaptic Activity of Neurons Derived from Human Pluripotent Stem Cells and Primary CNS Tissues
Scientific Poster
Significantly Greater Numbers of Neurons, Neurite Outgrowth and Branch Points in 21 Day Cultures of Primary Rat Cortical Neurons
Scientific Poster
Complete Serum-Free Culture Kit and Protocols for Culturing High Yields of Functional Mature Neurons from Primary Embryonic Mouse CNS Tissues

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Protocol for Plating and Culturing Primary Neurons with the SM1 Culture System

Primary rodent tissue dissociated in papain was plated in NeuroCult™ Neuronal Plating Medium, supplemented with NeuroCult™ SM1 Neuronal Supplement, L-Glutamine, and L-Glutamic Acid. On day 5, primary neurons were transitioned to BrainPhys™ Neuronal Medium, supplemented with NeuroCult™ SM1 Neuronal Supplement, by performing half-medium changes every 3 - 4 days.

Figure 2. The SM1 Culture System Supports Long-Term Culture of Rodent Neurons

Primary E18 rat cortical neurons were cultured in the SM1 Culture System. A large number of viable neurons are visible after (A) 21 and (B) 35 days, as demonstrated by their bright neuronal cell bodies, and extensive neurite outgrowth and branching. Neurons are evenly distributed over the culture surface with minimal cell clumping.

Figure 3. Pre- and Post-Synaptic Markers are Expressed in Rodent Neurons Cultured in the SM1 Culture System

Primary E18 rat cortical neurons were cultured in the SM1 Culture System. At 21 DIV, neurons are phenotypically mature, as indicated by the presence of an extensive dendritic arbor, and appropriate expression and localization of pre-synaptic synapsin (A,C; green) and post-synaptic PSD-95 (A,B; red) markers. Synapsin is concentrated in discrete puncta distributed along the somata and dendritic processes, as defined by the dendritic marker MAP2 (A,D; blue).

Figure 4. The SM1 Culture System Supports Increased Cell Survival

(A) Primary E18 rat cortical neurons were cultured in the SM1 Culture System or a Competitor Culture System for 21 days. Neurons cultured in the SM1 Culture System have a significantly higher number of viable cells compared to the competitor culture system (n = 4; mean ± 95% CI; *p < 0.05). (B) Primary E18 rat cortical neurons were cultured in Neurobasal® supplemented with NeuroCult™ SM1 Neuronal Supplement (SM1) or competitor B27-like supplements (Competitor 1,2,3) for 21 days. Cultures supplemented with NeuroCult™ SM1 Neuronal Supplement have an equal number of neurons compared to competitor-supplemented cultures. Bars represent standard error of mean.

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