NeuroCult™ Neuronal Plating Medium

Culture medium for plating dissociated primary tissue-derived neurons for improved survival

NeuroCult™ Neuronal Plating Medium

Culture medium for plating dissociated primary tissue-derived neurons for improved survival

NeuroCult™ Neuronal Plating Medium
100 mL
61 USD
Catalog # 05713

Culture medium for plating dissociated primary tissue-derived neurons for improved survival

Product Advantages


  • Optimized for the survival of primary tissue-derived neurons

  • Tested for compatibility with BrainPhys™ Neuronal Medium

Overview

NeuroCult™ Neuronal Plating Medium is a serum-free neuronal basal medium. It is designed for use with BrainPhys™ Neuronal Medium (Catalog #05790) for the plating and culture of primary tissue-derived neurons.
Subtype
Basal Media, Specialized Media
Cell Type
Neurons
Species
Mouse, Rat
Application
Cell Culture, Maintenance
Brand
NeuroCult
Area of Interest
Neuroscience
Formulation
Serum-Free

Data Figures

Figure 1. Protocol for Plating and Culturing Primary Neurons with the SM1 Culture System

Primary rodent tissue dissociated in papain was plated in NeuroCult™ Neuronal Plating Medium, supplemented with NeuroCult™ SM1 Neuronal Supplement, L-Glutamine, and L-Glutamic Acid. On day 5, primary neurons were transitioned to BrainPhys™ Neuronal Medium, supplemented with NeuroCult™ SM1 Neuronal Supplement, by performing half-medium changes every 3 - 4 days.

Figure 2. The SM1 Culture System Supports Long-Term Culture of Rodent Neurons

Primary E18 rat cortical neurons were cultured in the SM1 Culture System. A large number of viable neurons are visible after (A) 21 and (B) 35 days, as demonstrated by their bright neuronal cell bodies, and extensive neurite outgrowth and branching. Neurons are evenly distributed over the culture surface with minimal cell clumping.

Figure 3. Pre- and Post-Synaptic Markers are Expressed in Rodent Neurons Cultured in the SM1 Culture System

Primary E18 rat cortical neurons were cultured in the SM1 Culture System. At 21 DIV, neurons are phenotypically mature, as indicated by the presence of an extensive dendritic arbor, and appropriate expression and localization of pre-synaptic synapsin (A,C; green) and post-synaptic PSD-95 (A,B; red) markers. Synapsin is concentrated in discrete puncta distributed along the somata and dendritic processes, as defined by the dendritic marker MAP2 (A,D; blue).

Figure 4. The SM1 Culture System Supports Increased Cell Survival

(A) Primary E18 rat cortical neurons were cultured in the SM1 Culture System or a Competitor Culture System for 21 days. Neurons cultured in the SM1 Culture System have a significantly higher number of viable cells compared to the competitor culture system (n = 4; mean ± 95% CI; *p < 0.05). (B) Primary E18 rat cortical neurons were cultured in Neurobasal® supplemented with NeuroCult™ SM1 Neuronal Supplement (SM1) or competitor B27-like supplements (Competitor 1,2,3) for 21 days. Cultures supplemented with NeuroCult™ SM1 Neuronal Supplement have an equal number of neurons compared to competitor-supplemented cultures. Bars represent standard error of mean.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05713
Lot #
All
Language
English
Catalog #
05713
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
05713
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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