Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35
Overview
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35
|
Catalog #
60073, 60073.1 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488
|
Catalog #
60073AD, 60073AD.1 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Biotin
|
Catalog #
60073BT, 60073BT.1 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, FITC
|
Catalog #
60073FI |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, PE
|
Catalog #
60073PE, 60073PE.1 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35
|
Catalog #
60073, 60073.1 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488
|
Catalog #
60073AD, 60073AD.1 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Biotin
|
Catalog #
60073BT, 60073BT.1 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, FITC
|
Catalog #
60073FI |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet
|
Product Name
Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, PE
|
Catalog #
60073PE, 60073PE.1 |
Lot #
All |
Language
English |
Educational Materials (2)
Data and Publications
Data
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Catalog #60062) followed by a goat anti-mouse IgG antibody, FITC. (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70 (Catalog #60062), followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488, Catalog #60062AD). B) Human ES cells were cultured in mTeSR™1 on Corning Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488.
Figure 3. Data for Biotin-Conjugated
Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Biotin, Catalog #60062BT) followed by SAV APC.
Figure 4. Data for FITC-Conjugated
Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, FITC, Catalog #60062FI).
Figure 5. Data for PE-Conjugated
(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE, Catalog #60062PE). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE (Catalog #60062PE). Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
