Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35

Mouse monoclonal IgG3, kappa isotype control antibody

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Mouse monoclonal IgG3, kappa isotype control antibody
From: 59 USD

Overview Related Products Scientific Resources Data and Publications

Overview

The MG3-35 antibody (IgG3, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype:
Isotype Controls
Target Antigen:
IgG3 Isotype Control
Alternative Names:
Not applicable
Conjugation:
Alexa Fluor 488; Biotin; PE; Unconjugated
Host Species:
Mouse
Cell Type:
Other
Application:
Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Immunology
Clone:
MG3-35
Gene ID:
Not applicable
Isotype:
IgG3, kappa

Scientific Resources

Product Documentation

Educational Materials

(3)
Brochure
Complete Antibody Listing
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Data and Publications

Data

Data for unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Catalog #60062) followed by a goat anti-mouse IgG antibody, FITC. (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70 (Catalog #60062), followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488, Catalog #60062AD). B) Human ES cells were cultured in mTeSR™1 on Corning Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488.

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Biotin, Catalog #60062BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, FITC, Catalog #60062FI).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE, Catalog #60062PE). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE (Catalog #60062PE). Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.