Mitochondrial Superoxide Dye

Sensitive fluorescent dye that detects superoxide production in mitochondria

Mitochondrial Superoxide Dye

Sensitive fluorescent dye that detects superoxide production in mitochondria

From: 335 USD
Catalog #
100-0991_C
Sensitive fluorescent dye that detects superoxide production in mitochondria

What's Included

  • Mitochondrial Superoxide Dye (Catalog #100-0991)
    • Mitochondrial Superoxide Dye, 100 tests

Overview

Monitor superoxide production in the mitochondria of live cells using Mitochondrial Superoxide Dye. This fluorescent dye is a valuable tool for detecting mitochondrial superoxide production to investigate oxidative stress, and has been verified for detection using flow cytometry. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are free radicals derived from oxygen and nitrogen molecules.

Features:
• Excitation wavelength: 510 nm
• Emission wavelength: 580 nm
• Readily oxidized by superoxide but less likely to be oxidized by ROS or RNS
• Oxidized product is highly fluorescent in cells
• Rapidly and selectively targeted to the mitochondria
• High signal-to-noise ratio
• Verified for detection using flow cytometry

Mitochondrial Superoxide Dye selectively targets the mitochondria of living cells. This dye becomes oxidized when it reacts with superoxide, a ROS, producing a red fluorescence. In the absence of superoxide, there is no absorption and negligible fluorescence. Though Mitochondrial Superoxide Dye is readily oxidized by superoxide, it is resistant to oxidation by other ROS and RNS. The high signal-to-noise ratio makes Mitochondrial Superoxide Dye a suitable reagent for superoxide detection in biological systems such as cellular respiration (Li et al. Front Immunol, 2018).
Application
Flow Cytometry

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0991
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0991
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Figure 1. Mitochondrial Superoxide Dye Detects Formation of Superoxides After Antimycin A Treatment.

Flow cytometry analysis of Jurkat cells labeled with Mitochondrial Superoxide Dye after incubation in the presence or absence of antimycin A.

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