Human Recombinant NGF-beta, ACF

Nerve growth factor-beta, animal component-free

Human Recombinant NGF-beta, ACF

Nerve growth factor-beta, animal component-free

From: 401 USD
Catalog #
(Select a product)
Nerve growth factor-beta, animal component-free
Add to Wish List

Overview

Nerve growth factor (NGF)-beta is a prototypical member of the neurotrophin family and has a role in the survival and growth of neural cells, regulating cell growth, promoting differentiation into neurons, and neuron migration. The beta subtype of NGF is biologically active in comparison to the alpha-2 and gamma-2 subtypes. NGF-beta in its secreted form can bind to tyrosine kinase A (trkA) receptor with high affinity and to p75 (NTR) with low affinity (Levi & Alemà; Sofroniew et al.). NGF has been shown to possess pro-inflammatory and pro-fibrogenic properties (Micera et al.). It has also been shown that overexpression of NGF-beta promotes differentiation of bone marrow mesenchymal stem cells into neurons through regulation of AKT and MAPK pathways (Yuan et al.). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Alternative Names
Beta-nerve growth factor, beta-NGF, nerve growth factor (beta polypeptide)
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Formulation Category
Animal Component-Free
Molecular Weight
13.6 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant NGF-beta, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 60 hours in culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.31 ng/mL. (B) 1 μg of Human Recombinant NGF-beta, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78151.2, 78151.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78151.2, 78151.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.