Data and Publications
(A) The biological activity of Human Recombinant IGFBP-4 was tested by its ability to inhibit IGF-II induced proliferation of FDC-P1 cells.Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.05 μg/mL. (B) 5 μg of Human Recombinant IGFBP-4 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recmbinant IGFBP-4 has a predicted molecular mass of 26.8 kDa.