FITC-C6-DEVD-FMK

For detection of active caspase-3 in mammalian cells undergoing apoptosis

FITC-C6-DEVD-FMK

For detection of active caspase-3 in mammalian cells undergoing apoptosis

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For detection of active caspase-3 in mammalian cells undergoing apoptosis
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Overview

Efficiently detect active caspase-3 in mammalian cells undergoing apoptosis by using FITC-C6-DEVD-FMK. This reagent uses a quick and simple staining protocol that is highly sensitive and compatible with standard flow cytometers.

Caspase-3 is a proteolytic enzyme with a substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). FITC-C6-DEVD-FMK is a non-toxic, cell-permeable fluorescent substrate that irreversibly binds to activated caspase-3 in apoptotic cells. The FITC-labeled substrate can be detected with high sensitivity using flow cytometry.

Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases—caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). FITC-C6-DEVD-FMK is useful for screening caspase-3 inhibitors and quantifying apoptotic cells containing active caspase-3.
Application
Flow Cytometry
Area of Interest
Cancer
CAS Number
67-68-5
Molecular Weight
994.99 g/mol

Data Figures

Figure 1. FITC-C6-DEVD-FMK

Jurkat cells were treated with 0.2% DMSO (Control) or 20 µM camptothecin (CPT), and in the presence or absence of Z-VAD-FMK (a pan-caspase inhibitor). Active caspase-3/7 was detected by flow cytometry, using FITC-C6-DEVD-FMK. A representative plot of three independent experiments is depicted in the figure above.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
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Product Name
FITC-C6-DEVD-FMK
Catalog #
100-0924
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
FITC-C6-DEVD-FMK
Catalog #
100-0924
Lot #
All
Language
English