ES-Cult™ M3120

Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells

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ES-Cult™ M3120

Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells

40 mL
Catalog #03120
93 USD

Overview

ES-Cult™ M3120 is recommended as a base for the preparation of methylcellulose-based medium for the in vitro differentiation of mouse embryonic stem (ES) or induced pluripotent stem (iPS) cells into hematopoietic or endothelial progenitor cells. When supplemented with the appropriate additional components, ES-Cult™ M3120 can be used for the generation of embryoid bodies (EBs) from undifferentiated mouse ES or iPS cells.
Contains:
• 2.6% Methylcellulose
• Iscove’s MDM
Subtype:
Specialized Media; Semi-Solid Media
Cell Type:
Pluripotent Stem Cells
Species:
Mouse
Application:
Differentiation; Cell Culture
Brand:
ES-Cult
Area of Interest:
Stem Cell Biology
Formulation:
Defined; Serum-Free

Scientific Resources

Product Documentation

Educational Materials

(2)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications

(1)
Yearbook of Dermatology and Dermatologic Surgery 2008 DEC

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Yu J et al.

Abstract

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.