EasySep™ Non-Human Primate B Cell Isolation Kit
Immunomagnetic negative selection kit
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EasySep™ Non-Human Primate B Cell Isolation Kit -
RoboSep™ Non-Human Primate B Cell Isolation Kit -
Label for EasySep™ Non-Human Primate B Cell Isolation Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Anti-Human CD20 Antibody, Clone 2H7
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20
Overview
The EasySep™ Non-Human Primate B Cell Isolation Kit is designed to isolate untouched and highly purified B cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) in as little as 20 minutes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This kit has been verified for use with rhesus and cynomolgus macaques.
This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages:
⦁ Fast, easy to use, and column-free
⦁ Up to 95% purity
⦁ Untouched, viable cells
⦁ Up to 95% purity
⦁ Untouched, viable cells
Components:
- EasySep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0345)
- EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- EasySep™ Isolation Cocktail Enhancer, 1 mL
- RoboSep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0347)
- EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- EasySep™ Isolation Cocktail Enhancer, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Non-Human Primate
Sample Source:
PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology; Infectious Diseases
Related Products
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EasySep™ Non-Human Primate T Cell Isolation Kit
20-Minute cell isolation kit using immunomagnetic negative selection -
EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
25-Minute cell isolation kit using immunomagnetic negative selection -
EasySep™ Non-Human Primate Custom Enrichment Kit
Immunomagnetic negative selection kit -
EasySep™ Non-Human Primate Custom Positive Selection Kit
Immunomagnetic positive selection kit
Scientific Resources
Product Documentation
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Educational Materials
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. EasySep™ Non-Human Primate B Cell Isolation Kit
Starting with rhesus PBMCs, the B cell content (CD20+) of the isolated fraction is typically 91.4 ± 5.2% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.4% and 94.8%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.