EasySep™ Non-Human Primate B Cell Isolation Kit

Immunomagnetic negative selection kit

EasySep™ Non-Human Primate B Cell Isolation Kit

Immunomagnetic negative selection kit

From: 1,085 USD
Catalog #
100-0345_C
Immunomagnetic negative selection kit

Product Advantages


  • Fast, easy to use, and column-free

  • Up to 95% purity

  • Untouched, viable cells

What's Included

  • EasySep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0345)
    • EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Isolation Cocktail Enhancer, 1 mL
  • RoboSep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0347)
    • EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Isolation Cocktail Enhancer, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

The EasySep™ Non-Human Primate B Cell Isolation Kit is designed to isolate untouched and highly purified B cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) in as little as 20 minutes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit has been verified for use with rhesus and cynomolgus macaques.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Non-Human Primate
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology, Infectious Diseases

Data Figures

The content of rhesus macaque CD20+ B cells before and after isolation correspond to 10.4% and 94.8%, respectively.

Figure 1. EasySep™ Non-Human Primate B Cell Isolation Kit

Starting with rhesus PBMCs, the B cell content (CD20+) of the isolated fraction is typically 91.4 ± 5.2% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.4% and 94.8%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0345
Lot #
All
Language
English
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0347
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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