EasySep™ Non-Human Primate B Cell Isolation Kit

Immunomagnetic negative selection kit

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From: 975 USD

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Immunomagnetic negative selection kit
From: 975 USD

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Overview

The EasySep™ Non-Human Primate B Cell Isolation Kit is designed to isolate untouched and highly purified B cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) in as little as 20 minutes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages:
⦁ Fast, easy to use, and column-free
⦁ Up to 95% purity
⦁ Untouched, viable cells
Components:
  • EasySep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0345)
    • EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Isolation Cocktail Enhancer, 1 mL
  • RoboSep™ Non-Human Primate B Cell Isolation Kit (Catalog #100-0347)
    • EasySep™ Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • EasySep™ Isolation Cocktail Enhancer, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Non-Human Primate
Sample Source:
PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology; Infectious Diseases

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

The content of rhesus macaque CD20+ B cells before and after isolation correspond to 10.4% and 94.8%, respectively.

Figure 1. EasySep™ Non-Human Primate B Cell Isolation Kit

Starting with rhesus PBMCs, the B cell content (CD20+) of the isolated fraction is typically 91.4 ± 5.2% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.4% and 94.8%, respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.