EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

25-Minute cell isolation kit using immunomagnetic negative selection
Catalog #
19582_C
25-Minute cell isolation kit using immunomagnetic negative selection
From: 855 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This kit has been verified for use with rhesus and cynomolgus macaques.
Advantages
⦁ Fast, easy to use, and column-free
⦁ Up to 85% purity with high recovery
⦁ Untouched, viable cells
Components
  • EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
  • RoboSep™ Non-Human Primate CD4+ T Cell Isolation Kit (Catalog #19582RF)
    • EasySep™ Non-Human Primate CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D Magnetic Particles, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Non-Human Primate
Sample Source
PBMC, Peripheral Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
HIV, Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
Catalog #
19582, 19582RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
Catalog #
19582, 19582RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit
Catalog #
19582, 19582RF
Lot #
All
Language
English

Educational Materials (5)

Brochure
EasySep™ Cell Separation Technology
Brochure
EasySep™: Isolate Cells from Non-Human Primates
Brochure
Tools For Your Immunology Research
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

The content of rhesus macaque CD4+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the CD4+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 49.5% and 92.3%, respectively.

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