EasySep™ Mouse CD90.2 Positive Selection Kit II
Immunomagnetic positive selection kit
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EasySep™ Mouse CD90.2 Positive Selection Kit II -
RoboSep™ Mouse CD90.2 Positive Selection Kit II -
Label for EasySep™ Mouse CD90.2 Positive Selection Kit II
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Mouse CD90.2 Positive Selection Kit II is designed to isolate CD90.2+ (Thy1.2+) cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.2 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
This product replaces the EasySep™ Mouse CD90.2 Positive Selection Kit (Catalog #18751) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
This product replaces the EasySep™ Mouse CD90.2 Positive Selection Kit (Catalog #18751) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components:
- EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951)
- EasySep™ Mouse CD90.2 Positive Selection II Component A, 0.5 mL
- EasySep™ Mouse CD90.2 Positive Selection II Component B, 0.5 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- RoboSep™ Empty Vial
- RoboSep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951RF)
- EasySep™ Mouse CD90.2 Positive Selection II Component A, 0.5 mL
- EasySep™ Mouse CD90.2 Positive Selection II Component B, 0.5 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- RoboSep™ Empty Vial
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells
Species:
Mouse
Sample Source:
Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
Related Products
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EasySep™ Mouse T Cell Isolation Kit
15-Minute cell isolation kit using immunomagnetic negative selection -
EasySep™ Mouse CD90.1 Positive Selection Kit
Immunomagnetic positive selection cell isolation kit
Labeling Antibodies
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Anti-Mouse CD3e Antibody, Clone 145-2C11
Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
Educational Materials
(8)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Typical EasySep™ CD90.2 Positive Selection II Profile
Starting with mouse splenocytes, the CD90.2+ cell content of the isolated fraction is typically 95.8 ± 1.5% (mean ± SD using the purple EasySep™ Magnet).
Publications
(1)
Frontiers in immunology 2019
Macrophage Coordination of the Interferon Lambda Immune Response.
Abstract
Abstract
Lambda interferons (IFN-$\lambda$s) are a major component of the innate immune defense to viruses, bacteria, and fungi. In human liver, IFN-$\lambda$ not only drives antiviral responses, but also promotes inflammation and fibrosis in viral and non-viral diseases. Here we demonstrate that macrophages are primary responders to IFN-$\lambda$, uniquely positioned to bridge the gap between IFN-$\lambda$ producing cells and lymphocyte populations that are not intrinsically responsive to IFN-$\lambda$. While CD14+ monocytes do not express the IFN-$\lambda$ receptor, IFNLR1, sensitivity is quickly gained upon differentiation to macrophages in vitro. IFN-$\lambda$ stimulates macrophage cytotoxicity and phagocytosis as well as the secretion of pro-inflammatory cytokines and interferon stimulated genes that mediate immune cell chemotaxis and effector functions. In particular, IFN-$\lambda$ induced CCR5 and CXCR3 chemokines, stimulating T and NK cell migration, as well as subsequent NK cell cytotoxicity. Using immunofluorescence and cell sorting techniques, we confirmed that human liver macrophages expressing CD14 and CD68 are highly responsive to IFN-$\lambda$ ex vivo. Together, these data highlight a novel role for macrophages in shaping IFN-$\lambda$ dependent immune responses both directly through pro-inflammatory activity and indirectly by recruiting and activating IFN-$\lambda$ unresponsive lymphocytes.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.