EasySep™ Human Naïve B Cell Isolation Kit
9-Minute cell isolation kit using immunomagnetic negative selection
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In-lab demonstration
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EasySep™ MagnetMagnet for column-free immunomagnetic separation
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EasySep™ BufferCell separation buffer
Overview
Data Figures
Starting with human PBMCs, the naïve B cell (CD3-CD19+CD27-) content of the isolated fraction is typically 94.9 ± 2.2% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 7.1% and 97.7%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (11)
Frequently Asked Questions
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
