EasySep™ Human CD45 Depletion Kit II
Immunomagnetic depletion cell isolation kit
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EasySep™ Human CD45 Depletion Kit II -
EasySep™ Human CD45 Depletion Kit II -
Label for EasySep™ Human CD45 Depletion Kit II
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Human CD45 Depletion Kit II is designed to deplete CD45+ cells from fresh or previously frozen human peripheral blood mononuclear cells. Unwanted cells are targeted with antibody complexes recognizing CD45 and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off. The CD45 antigen is expressed on all hematopoietic cells except erythrocytes and platelets.
This product replaces the EasySep™ Human CD45 Depletion Kit (Catalog #18259) for even faster cell isolations.
This product replaces the EasySep™ Human CD45 Depletion Kit (Catalog #18259) for even faster cell isolations.
Advantages:
• Fast, easy-to-use and column-free
• Up to 4 log depletion of CD45+ cells
• Isolated cells are untouched
• Up to 4 log depletion of CD45+ cells
• Isolated cells are untouched
Components:
- EasySep™ Human CD45 Depletion Kit II (Catalog #17898)
- EasySep™ Human CD45 Depletion Cocktail II, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- RoboSep™ Human CD45 Depletion Kit II (Catalog #17898RF)
- EasySep™ Human CD45 Depletion Cocktail II, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Cancer Cells and Cell Lines
Species:
Human
Sample Source:
PBMC
Selection Method:
Depletion
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Cancer Research; Stem Cell Biology; Immunology
Related Products
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RosetteSep™ Human CD45 Depletion Cocktail
Immunodensity depletion cocktail
Labeling Antibodies
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Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45 -
Anti-Human CD45 Antibody, Clone 2D1
Mouse monoclonal IgG1 antibody against human CD45
Scientific Resources
Product Documentation
Document Type
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Catalog #
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Language
Educational Materials
(5)
Data and Publications
Data
In the example above, CAMA cells were seeded into PBMCs at a starting frequency of 1.1% (98.9% CD45+; gated on DRAQ5™ for nucleated cells). The CAMA cell (EpCAM+) content of the depleted fraction is 98%, which is a 4.0 log depletion of CD45+ cells.
NOTE: EpCAM is an antibody against an epithelial cell surface antigen expressed on CAMA cells.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.